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JXB Advance Access originally published online on July 10, 2006
Journal of Experimental Botany 2006 57(11):2613-2625; doi:10.1093/jxb/erl025
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

A metabonomic study of transgenic maize (Zea mays) seeds revealed variations in osmolytes and branched amino acids

Cesare Manetti*, Cristiano Bianchetti, Lorena Casciani, Cecilia Castro, Maria Enrica Di Cocco, Alfredo Miccheli, Mario Motto and Filippo Conti

Dipartimento di Chimica, Università degli studi di Roma ‘La Sapienza’, Piazzale Aldo Moro 5, I-00185 Roma, Italy and Istituto Sperimentale per la Cerealicoltura, Sez. Bergamo, Via Stezzano 24, I-24126 Bergamo, Italy

*To whom correspondence should be addressed. E-mail: c.manetti{at}caspur.it

The aim of the research was to investigate metabolic variations associated with genetic modifications in the grains of Zea mays using metabonomic techniques. With this in mind, the non-targeted characteristic of the technique is useful to identify metabolites peculiar to the genetic modification and initially undefined. The results obtained showed that the genetic modification, introducing Cry1Ab gene expression, induces metabolic variations involving the primary nitrogen pathway. Concerning the methodological aspects, the experimental protocol used has been applied in this field for the first time. It consists of a combination of partial least square-discriminant analysis and principal component analysis. The most important metabolites for discrimination were selected and the metabolic correlations linking them are identified. Principal component analysis on selected signals confirms metabolic variations, highlighting important details about the changes induced on the metabolic network by the presence of a Bt transgene in the maize genome.

Key words: GMO, metabolomics, metabonomics, multivariate analysis, NMR, Zea mays


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