Skip Navigation


JXB Advance Access originally published online on July 13, 2006
Journal of Experimental Botany 2006 57(11):2719-2734; doi:10.1093/jxb/erl034
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
57/11/2719    most recent
erl034v2
erl034v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lasseur, B.
Right arrow Articles by Prud'homme, M.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lasseur, B.
Right arrow Articles by Prud'homme, M.-P.
Agricola
Right arrow Articles by Lasseur, B.
Right arrow Articles by Prud'homme, M.-P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.)

Bertrand Lasseur1, Jérémy Lothier1, Abdelmadjid Djoumad2, Barbara De Coninck4, Sjef Smeekens3, André Van Laere4, Annette Morvan-Bertrand1, Wim Van den Ende4 and Marie-Pascale Prud'homme1,*

1UMR INRA-UCN 950 EVA Ecophysiologie Végétale, Agronomie et Nutritions NCS, Université de Caen, Esplanade de la Paix, F-14032 Caen cedex, France
2Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbooke, QC, Canada
3Department of Molecular Plant Physiology, University Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
4Department of Biology, Laboratory for Molecular Plant Physiology, Botany Institute, KULeuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium

*To whom correspondence should be addressed. E-mail: marie-pascale.prudhomme{at}unicaen.fr

Fructans are the main storage compound in Lolium perenne. To account for the prevailing neokestose-based fructan synthesis in this species, a cDNA library of L. perenne was screened by using the onion (Allium cepa) fructan:fructan 6G-fructosyltransferase (6G-FFT) as a probe. A full length Lp6G-FFT clone was isolated with significant homologies to vacuolar type fructosyltransferases and invertases. The functionality of the cDNA was tested by heterologous expression in Pichia pastoris. The recombinant protein demonstrated both 6G-FFT and fructan:fructan 1-fructosyltransferase activities (1-FFT) with a maximum 6G-FFT/1-FFT ratio of two. The activity of 6G-FFT was investigated with respect to developmental stage, tissue distribution, and alterations in carbohydrate status expression and compared to sucrose:sucrose 1-fructosyltransferase (1-SST). Lp6G-FFT and Lp1-SST were predominantly expressed in the basal part of elongating leaves and leaf sheaths. Expression of both genes declined along the leaf axis, in parallel with the spatial occurrence of fructan and fructosyltransferase activities. Surprisingly, Lp6G-FFT was highly expressed in photosynthetically active tissues where very low extractable fructosyltransferase activity and fructan amounts were detected, suggesting a post-transcriptional regulation of expression. Lp6G-FFT gene expression increased only in elongating leaves following similar increases of sucrose content in blades, sheaths, and elongating leaf bases. Regulation of Lp6G-FFT gene expression depends on the tissue according to its sink–source status.

Key words: Fructan, fructan:fructan 6G-fructosyltransferase, gene expression, heterologous expression, Lolium perenne, Pichia pastoris, sucrose:sucrose 1-fructosyltransferase


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
R. Valluru and W. Van den Ende
Plant fructans in stress environments: emerging concepts and future prospects
J. Exp. Bot., August 1, 2008; 59(11): 2905 - 2916.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G.-P. Xue, C. L. McIntyre, C. L.D. Jenkins, D. Glassop, A. F. van Herwaarden, and R. Shorter
Molecular Dissection of Variation in Carbohydrate Metabolism Related to Water-Soluble Carbohydrate Accumulation in Stems of Wheat
Plant Physiology, February 1, 2008; 146(2): 441 - 454.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Lothier, B. Lasseur, K. Le Roy, A. Van Laere, M.-P. Prud'homme, P. Barre, W. Van den Ende, and A. Morvan-Bertrand
Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
J. Exp. Bot., June 1, 2007; 58(8): 1969 - 1983.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.