Skip Navigation


JXB Advance Access originally published online on August 7, 2006
Journal of Experimental Botany 2006 57(12):3145-3155; doi:10.1093/jxb/erl076
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/12/3145    most recent
erl076v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lannoo, N.
Right arrow Articles by Van Damme, E. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lannoo, N.
Right arrow Articles by Van Damme, E. J. M.
Agricola
Right arrow Articles by Lannoo, N.
Right arrow Articles by Van Damme, E. J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

The presence of jasmonate-inducible lectin genes in some but not all Nicotiana species explains a marked intragenus difference in plant responses to hormone treatment

Nausicaä Lannoo, Willy J. Peumans and Els J. M. Van Damme*

Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Gent, Belgium

*To whom correspondence should be addressed. E-mail: ElsJM.VanDamme{at}UGent.be

Tobacco (Nicotiana tabacum L. cv Samsun NN) leaves accumulate a cytoplasmic/nuclear lectin, called Nictaba, in response to methyl jasmonate. To check whether, and if so to what extent, the specific induction of this lectin applies to related species, a collection of 19 Nicotiana species—covering 12 Nicotiana sections and eight Nicotiana tabacum cultivars—was screened for their capability to synthesize the jasmonate-inducible lectin. Protein analyses by agglutination assays and western blot confirmed that only nine out of the 19 species examined synthesize lectin after jasmonate treatment. Remarkably, all allotetraploid cultivars of the N. tabacum L. species tested express the lectin after jasmonate treatment. PCR analyses demonstrated that all responsive species possess one or more lectin genes, whereas no lectin gene(s) could be traced in the non-responding species. The number of introns present in the lectin genes varies between zero and two. Four tobacco species/cultivars contain both intronless Nictaba genes as well as lectin genes with introns. These findings provide the first firm evidence for a striking intragenus difference with respect to the activation of a well-defined jasmonate-inducible gene that can be correlated with the presence/absence of orthologous genes in the genomes of closely related species from a single plant genus. In addition, the differential response of closely related tobacco species illustrates that in the field of plant hormone research, care must be taken when extrapolating results obtained with a particular model system to other—even taxonomically closely related—species.

Key words: Gene loss, inducible protein, jasmonate, lectin gene, Nicotiana, Nictaba, tobacco


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Schwachtje and I. T. Baldwin
Why Does Herbivore Attack Reconfigure Primary Metabolism?
Plant Physiology, March 1, 2008; 146(3): 845 - 851.
[Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Lannoo, G. Vandenborre, O. Miersch, G. Smagghe, C. Wasternack, W. J. Peumans, and E. J. M. Van Damme
The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin
Plant Cell Physiol., August 1, 2007; 48(8): 1207 - 1218.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.