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JXB Advance Access originally published online on September 12, 2006
Journal of Experimental Botany 2006 57(14):3553-3562; doi:10.1093/jxb/erl108
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Sethoxydim affects lipid synthesis and acetyl-CoA carboxylase activity in soybean

Aicha Belkebir1, Rosine De Paepe2,*, Antoine Trémolières2, Fatiha Aïd1 and Ghouziel Benhassaine-Kesri3

1Physiologie Végétale/LBPO, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne, BP 39, El Alia, Bab Ezzouar, Alger, Algérie
2Institut de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris-Sud, F-91405 Orsay, France
3Université Pierre et Marie Curie-Paris 6, FRE 2846, PCMP, Ivry-sur-Seine, F-94200 France

* To whom correspondence should be addressed. E-mail: depaepe{at}ibp.u-psud.fr

With rare exceptions, dicot plastids have been reported to contain only a multisubunit (prokaryotic) form of acetyl-coA carboxylase (ACCase), the first committed step of lipid biosynthesis. The sensitivity of most monocots to cyclohexanediones (CHDs) such as sethoxydim, has been shown to be associated with the presence in their plastids of a multifunctional (eukaryotic) form of ACCase. Little is known about the effects of sethoxydim on lipid metabolism and ACCase activity in dicots. Here it is shown that foliar lipid biosynthesis is differentially affected by the herbicide treatment in two dicot species, Nicotiana sylvestris (wild tobacco) and Glycine max (soybean). In N. sylvestris, the total lipid content of neoformed leaves harvested 2 weeks after the sethoxydim treatment was unaffected by doses of up to 10–3 M sethoxydim. In soybean, lipid content decreased by 45% when 10–5 M sethoxydim was used, and this was associated with a 30% reduction in fatty acid synthesis activity. ACCase activity of soybean plastidial preparations was 60% reduced in the presence of sethoxydim, whereas that of N. sylvestris was unaffected. Finally, the presence of a biotinylated 220 kDa polypeptide, corresponding in size to multifunctional ACCase, was observed in soybean plastids. Possible relationships between sensitivity of plastidial soybean ACCase towards sethoxydim, plastidial protein content, and altered de novo lipid biosynthesis in herbicide-treated plants are discussed.

Key words: Acetyl-CoA carboxylase (ACCase), fatty acid synthesis, plant lipid metabolism, sethoxydim


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