Skip Navigation


JXB Advance Access originally published online on October 18, 2006
Journal of Experimental Botany 2006 57(15):4015-4023; doi:10.1093/jxb/erl172
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/15/4015    most recent
erl172v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Schaarschmidt, S.
Right arrow Articles by Hause, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaarschmidt, S.
Right arrow Articles by Hause, B.
Agricola
Right arrow Articles by Schaarschmidt, S.
Right arrow Articles by Hause, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Arbuscular mycorrhiza induces gene expression of the apoplastic invertase LIN6 in tomato (Lycopersicon esculentum) roots

Sara Schaarschmidt1, Thomas Roitsch2 and Bettina Hause1,*

1Leibniz-Institut für Pflanzenbiochemie (IPB), Weinberg 3, D-06120 Halle (Saale), Germany
2Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany

* To whom correspondence should be addressed. E-mail: bhause{at}ipb-halle.de

Extracellular invertases are suggested to play a crucial role in the arbuscular mycorrhiza (AM) symbiosis to fulfil the increased sink function of the mycorrhizal root and the supply of the obligate biotrophic AM fungus with hexoses. In tomato (Lycopersicon esculentum), LIN6 represents an apoplastic invertase which is described as a key enzyme in establishing and maintaining sink metabolism. In this study, transcript levels of LIN6 were analysed in tomato roots colonized with the AM fungus Glomus intraradices. Using real-time RT–PCR, a nearly 3-fold increase in LIN6 mRNA levels was detected at late stages of mycorrhization (11 weeks after inoculation). A 1.8-fold induction could already be achieved at earlier stages (5 weeks after inoculation) using higher inoculum concentrations, whereas wounding of non-mycorrhizal roots resulted in up to 12-fold enhanced LIN6 transcripts. As revealed by in situ hybridization, the expression of LIN6 upon mycorrhization was specifically restricted to colonized cells and to the central cylinder. Such a strongly localized pattern due to mycorrhizal cells and to the central core could also be shown for promoter activity using transgenic Nicotiana tabacum plants expressing the gene coding for ß-glucuronidase under the control of the LIN6 promoter. The moderate induction of LIN6 expression in mycorrhizal tomato roots compared with stress-stimulated induction suggested a fine-tuning in the activation of sink metabolism in the mutualistic interaction, avoiding stress-induced defence reactions.

Key words: Apoplastic invertase LIN6, arbuscular mycorrhiza, in situ hybridization, Lycopersicon, real-time RT-PCR, transgenic Nicotiana


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. I. Zanor, S. Osorio, A. Nunes-Nesi, F. Carrari, M. Lohse, B. Usadel, C. Kuhn, W. Bleiss, P. Giavalisco, L. Willmitzer, et al.
RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility
Plant Physiology, July 1, 2009; 150(3): 1204 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Guether, B. Neuhauser, R. Balestrini, M. Dynowski, U. Ludewig, and P. Bonfante
A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi
Plant Physiology, May 1, 2009; 150(1): 73 - 83.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. K. Proels and T. Roitsch
Extracellular invertase LIN6 of tomato: a pivotal enzyme for integration of metabolic, hormonal, and stress signals is regulated by a diurnal rhythm
J. Exp. Bot., April 1, 2009; 60(6): 1555 - 1567.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Nehls
Mastering ectomycorrhizal symbiosis: the impact of carbohydrates
J. Exp. Bot., March 1, 2008; 59(5): 1097 - 1108.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Berger, A. K. Sinha, and T. Roitsch
Plant physiology meets phytopathology: plant primary metabolism and plant pathogen interactions
J. Exp. Bot., December 1, 2007; 58(15-16): 4019 - 4026.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Schaarschmidt, M.-C. Gonzalez, T. Roitsch, D. Strack, U. Sonnewald, and B. Hause
Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity
Plant Physiology, April 1, 2007; 143(4): 1827 - 1840.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.