Skip Navigation


JXB Advance Access originally published online on October 30, 2006
Journal of Experimental Botany 2006 57(15):4079-4088; doi:10.1093/jxb/erl175
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/15/4079    most recent
erl175v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Dorion, S.
Right arrow Articles by Rivoal, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorion, S.
Right arrow Articles by Rivoal, J.
Agricola
Right arrow Articles by Dorion, S.
Right arrow Articles by Rivoal, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Autophosphorylation of Solanum chacoense cytosolic nucleoside diphosphate kinase on Ser117

Sonia Dorion1, France Dumas2 and Jean Rivoal1,*

1IRBV, Université de Montréal, 4101 Rue Sherbrooke est, Montréal, QC, H1X 2B2 Canada
2Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montréal, QC, H4P 2R2 Canada

* To whom correspondence should be addressed. E-mail: jean.rivoal{at}umontreal.ca

NDPK catalyses the interconversion of NTPs and NDPs using a phosphohistidine intermediate as part of its catalytic site. Recombinant Solanum chacoense cytosolic NDPK incubated with [{gamma}-32P]ATP was allowed to autophosphorylate and 32P-labelled P-Ser was identified in an acid hydrolysate of the protein by two-dimensional TLC. Further analysis of 32P-labelled recombinant NDPK by tryptic digestion followed by automated Edman sequencing of the radioactive peptide allowed the identification of a single and conserved P-Ser residue at position 117. Analysis of site-directed mutants where Ser117 was substituted to Asp indicated that the presence of a negative charge at position 117 dramatically lowered the enzyme's catalytic efficiency. Ser autophosphorylation was markedly reduced with increasing ADP concentrations in the autophosphorylation assay. These findings provide evidence that autophosphorylation of cytosolic NDPK on Ser117 could constitute a regulatory mechanism for this important enzyme and that autophosphorylation of Ser117 is modulated by NDP availability.

Key words: Enzymology, nucleoside diphosphate kinase, plant, phosphoamino acid, phosphorylation, protein sequencing, site-directed mutagenesis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.