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JXB Advance Access originally published online on November 29, 2005
Journal of Experimental Botany 2006 57(2):235-247; doi:10.1093/jxb/erj005
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Exploitation of genomic sequences in a systematic analysis to access how cyanobacteria sense environmental stress

Norio Murata1,* and Iwane Suzuki2

1National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan
2Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba 305-8572, Japan

* To whom correspondence should be addressed. E-mail: murata{at}nibb.ac.jp

The perception and subsequent transduction of environmental signals are primary events in the acclimation of living organisms to changes in their environment. Many of the molecular sensors and transducers of environmental stress cannot be identified by traditional and conventional methods. Therefore, the genomic information has been exploited in a systematic approach to this problem, performing systematic mutagenesis of potential sensors and transducers, namely, histidine kinases and response regulators, respectively, in combination with DNA microarray analysis, to examine the genome-wide expression of genes in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Using targeted mutagenesis, 44 out of the 47 histidine kinases and 42 out of the 45 response regulators of this organism have successfully been inactivated. The resultant mutant libraries were screened by genome-wide DNA microarray analysis and by slot-blot hybridization analysis under various stress and non-stress conditions. Histidine kinases have been identified that perceive and transduce signals of low-temperature, hyperosmotic, and salt stress, as well as manganese deficiency.

Key words: Hyperosmotic stress, histidine kinase, low-temperature stress, Mn deficiency, response regulator, salt stress, sensor of environmental stress, signal transduction, Synechocystis sp. PCC 6803, two-component system


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