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JXB Advance Access originally published online on December 23, 2005
Journal of Experimental Botany 2006 57(3):547-557; doi:10.1093/jxb/erj035
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

The two senescence-related markers, GS1 (cytosolic glutamine synthetase) and GDH (glutamate dehydrogenase), involved in nitrogen mobilization, are differentially regulated during pathogen attack and by stress hormones and reactive oxygen species in Nicotiana tabacum L. leaves

Karine Pageau1, Michèle Reisdorf-Cren1,2, Jean-François Morot-Gaudry1 and Céline Masclaux-Daubresse1,*

1INRA Unité de Nutrition Azotée des Plantes, Route de Saint Cyr, F-78 026 Versailles Cedex, France
2Université de Versailles-Saint Quentin, Département de Biologie, 45 avenue des Etats unis, F-78 035 Versailles Cedex, France

* To whom correspondence should be addressed. E-mail: masclaux{at}versailles.inra.fr

To investigate the role of stress in nitrogen management in plants, the effect of pathogen attack, elicitors, and phytohormone application on the expression of the two senescence-related markers GS1 (cytosolic glutamine synthetase EC 6.3.1.2 [EC] ) and GDH (glutamate dehydrogenase, EC 1.4.1.2 [EC] ) involved in nitrogen mobilization in senescing leaves of tobacco (Nicotiana tabacum L.) plants, was studied. The expression of genes involved in primary nitrogen assimilation such as GS2 (chloroplastic glutamine synthetase) and Nia (nitrate reductase, EC 1.6.1.1 [EC] ) was also analysed. The Glubas gene, coding a ß-1,3-glucanase, was used as a plant-defence gene control. As during natural senescence, the expression of GS2 and Nia was repressed under almost all stress conditions. By contrast, GS1 and GDH mRNA accumulation was increased. However, GS1 and GDH showed differential patterns of expression depending on the stress applied. The expression of GS1 appeared more selective than GDH. Results indicate that the GDH and GS1 genes involved in leaf senescence are also a component of the plant defence response during plant–pathogen interaction. The links between natural plant senescence and stress-induced senescence are discussed, as well as the potential role of GS1 and GDH in a metabolic safeguard process.

Key words: Glutamate dehydrogenase, glutamine synthetase, leaf senescence, nitrate reductase, nitrogen metabolism, plant–pathogen interaction, stress


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