JXB Advance Access originally published online on March 2, 2006
Journal of Experimental Botany 2006 57(5):1119-1128; doi:10.1093/jxb/erj093
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RESEARCH PAPER |
Identification of plant stress-responsive determinants in arabidopsis by large-scale forward genetic screens
1Department of Horticultural Science and Vegetable and Fruit Improvement Center, 2133 Texas A&M University, College Station, TX 77843-2133, USA
2Center for Plant Environmental Stress Physiology, 625 Agriculture Mall Drive, Purdue University, West Lafayette, IN 47907-2010, USA
* To whom correspondence should be addressed. E-mail: koiwa{at}neo.tamu.edu
All plants sense and adapt to adverse environmental conditions, however, crop plants exhibit less genetic diversity for abiotic stress tolerance than do wild relatives indicating that a genetic basis exists for stress adaptability. Model plant genetic systems and the plethora of molecular genetic resources that are currently available are greatly enhancing our ability to identify abiotic stress-responsive genetic determinants. Forward genetic screens of T-DNA mutagenized Arabidopsis thaliana populations in the genetic background of ecotypes C24RD29a-LUC and Col-0 gl1 sos3-1 were carried out to begin an exhaustive search for such determinants. The C24RD29a-LUC screens identified mutants with altered salt/osmotic stress sensitivity or mutants with altered expression of the salt/osmotic/cold/ABA-responsive RD29a gene. Also, mutations that alter the NaCl sensitivity of sos3-1 were screened for potential genetic suppressors or enhancers of salt-stress responses mediated by SOS3. In total, more than 250 000 independent insertion lines were screened and greater than 200 individual mutants that exhibited altered stress/ABA responses were recovered. Although several of these mutants have been reported, most have not yet been studied in detail. Notable examples include novel alleles of SOS1 and mutations to genes encoding the STT3a subunit of the oligosaccharyltransferase, syntaxin, RNA polymerase II CTD phosphatases, transcription factors, ABA biosynthetic enzyme, Na+ transporter HKT1, and SUMO E3 ligase. The stress-specific phenotypes of mutations to genes that are involved in many basic cellular functions provide indication of the wide range of control mechanisms in cellular homeostasis that are involved in stress adaptation.
Key words: CCD imaging, osmotic stress, RNA polymerase II, salinity, T-DNA tagging, transcription
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