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JXB Advance Access originally published online on March 23, 2006
Journal of Experimental Botany 2006 57(6):1413-1421; doi:10.1093/jxb/erj121
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Society for Experimental Biology are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Diurnal changes in polyamine content, arginine and ornithine decarboxylase, and diamine oxidase in tobacco leaves

Lenka Gemperlová1,2, Marie Nováková1,3, Radomíra Vanková1, Josef Eder1 and Milena Cvikrová1,*

1Institute of Experimental Botany, Academy of Sciences of Czech Republic, Rozvojová 135, 165 02 Prague 6, Czech Republic
2Department of Plant Physiology, Faculty of Sciences, Charles University, Vinicná 5, 128 44 Prague 2, Czech Republic
3Department of Biochemistry, Faculty of Sciences, Charles University, Hlavova 2030/8, 128 44 Prague 2, Czech Republic

* To whom correspondence should be addressed. E-mail: cvikrova{at}ueb.cas.cz

Changes in the contents of polyamines (PAs) in tobacco leaves (Nicotiana tabacum L. cv. Wisconsin 38) grown under 16 h photoperiod were correlated with arginine and ornithine decarboxylase (EC 4.1.1.19 [EC] and EC 4.1.1.17 [EC] ) and diamine oxidase (EC 1.4.3.6 [EC] ) activities. The maximum of free and soluble conjugated forms of PAs occurred 1–2 h after the middle of the light period and was followed by two distinct peaks at the end of the light and at the beginning of the dark phase. Putrescine was the most abundant and cadaverine the least abundant PA in both free and PCA-soluble forms. However, cadaverine was predominant in PCA-insoluble conjugates, followed by putrescine, spermidine, and spermine. Both arginine and ornithine decarboxylases are involved in putrescine biosynthesis in tobacco leaves. Light dramatically stimulated the activity of ornithine decarboxylase, while no photoinduction of arginine decarboxylase activity was observed. Ornithine decarboxylase was found mainly in the particulate fraction. Only one peak, just after light induction, occurred in the cytosolic fraction, with 35% of the total ornithine decarboxylase activity. By contrast, the total arginine decarboxylase activity was equally divided between the soluble and pellet fractions. A sharp increase in diamine oxidase activity occurred 1 h after exposure to light, concomitant with the light-induced increase in ornithine decarboxylase activity. After a decline, diamine oxidase activity increased again, together with the rise in the amount of free Put. The roles of both conjugation of PAs with hydroxycinnamic acids and oxidative degradation of putrescine in maintaining free PA levels during the 24 h light/dark cycle are discussed. The presented results have shown that the parameters studied here followed rhythmical changes and were not only affected by light.

Key words: Arginine decarboxylase, diamine oxidase, ornithine decarboxylase, polyamines, tobacco


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