JXB Advance Access originally published online on March 17, 2006
Journal of Experimental Botany 2006 57(6):1423-1430; doi:10.1093/jxb/erj122
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RESEARCH PAPER |
Hypoxia interferes with ABA metabolism and increases ABA sensitivity in embryos of dormant barley grains
1IFEVA-Cátedra de Cerealicultura Facultad de Agronomia, Universidad de Buenos Aires/CONICET, Av. San Martin 4453, 1417 Buenos Aires, Argentina
2Université Pierre et Marie Curie-Paris 6, Physiologie Végétale Appliquée, EA 2388 Physiologie des semences, Site d'Ivry, Boîte 152, 4 Place Jussieu, F-75252 Paris cedex 05, France
* To whom correspondence should be addressed. E-mail: benech{at}ifeva.edu.ar
Two mechanisms have been suggested as being responsible for dormancy in barley grain: (i) ABA in the embryo, and (ii) limitation of oxygen supply to the embryo by oxygen fixation as a result of the oxidation of phenolic compounds in the glumellae. The aim of the present work was to investigate whether hypoxia imposed by the glumellae interferes with ABA metabolism in the embryo, thus resulting in dormancy. In dormant and non-dormant grains incubated at 20 °C and in non-dormant grains incubated at 30 °C (i.e. when dormancy is not expressed), ABA content in the embryo decreased dramatically during the first 5 h of incubation before germination was detected. By contrast, germination of dormant grains was less than 2% within 48 h at 30 °C and embryo ABA content increased during the first hours of incubation and then remained 2–4 times higher than in embryos from grains in which dormancy was not expressed. Removal of the glumellae allowed germination of dormant grains at 30 °C and the embryos did not display the initial increase in ABA content. Incubation of de-hulled grains under 5% oxygen to mimic the effect of glumellae, restored the initial increase ABA in content and completely inhibited germination. Incubation of embryos isolated from dormant grains, in the presence of a wide range of ABA concentrations and under various oxygen tensions, revealed that hypoxia increased embryo sensitivity to ABA by 2-fold. This effect was more pronounced at 30 °C than at 20 °C. Furthermore, when embryos from dormant grains were incubated at 30 °C in the presence of 10 µM ABA, their endogenous ABA content remained constant after 48 h of incubation under air, while it increased dramatically in embryos incubated under hypoxia, indicating that the apparent increase in embryo ABA responsiveness induced by hypoxia was, in part, mediated by an inability of the embryo to inactivate ABA. Taken together these results suggest that hypoxia, either imposed artificially or by the glumellae, increases embryo sensitivity to ABA and interferes with ABA metabolism.
Key words: Abscisic acid, barley, glumellae, hypoxia, seed dormancy
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