Skip Navigation


JXB Advance Access originally published online on April 4, 2006
Journal of Experimental Botany 2006 57(8):1785-1793; doi:10.1093/jxb/erj175
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/8/1785    most recent
erj175v2
erj175v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Barroso, J. B
Right arrow Articles by del Río, L. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barroso, J. B
Right arrow Articles by del Río, L. A
Agricola
Right arrow Articles by Barroso, J. B
Right arrow Articles by del Río, L. A
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress*

Juan B Barroso1, Francisco J Corpas2,{dagger}, Alfonso Carreras1, María Rodríguez-Serrano2, Francisco J Esteban1, Ana Fernández-Ocaña1, Mounira Chaki1, María C Romero-Puertas2, Raquel Valderrama1, Luisa M Sandalio2 and Luis A del Río2

1Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (Estación Experimental del Zaidín), Área de Bioquímica y Biología Molecular, Universidad de Jaén, E- 23071 Jaén, Spain
2Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain

{dagger}To whom correspondence should be addressed. E-mail: javier.corpas{at}eez.csic.es

S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 µM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress.

Key words: Abiotic stress, collenchyma, formaldehyde dehydrogenase, nitric oxide, reactive nitrogen species, RNS, S-nitrosoglutathione, S-nitrosoglutathione reductase, signalling


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Rodriguez-Serrano, M. C. Romero-Puertas, D. M. Pazmino, P. S. Testillano, M. C. Risueno, L. A. del Rio, and L. M. Sandalio
Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and Calcium
Plant Physiology, May 1, 2009; 150(1): 229 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Chaki, A. M. Fernandez-Ocana, R. Valderrama, A. Carreras, F. J. Esteban, F. Luque, M. V. Gomez-Rodriguez, J. C. Begara-Morales, F. J. Corpas, and J. B. Barroso
Involvement of Reactive Nitrogen and Oxygen Species (RNS and ROS) in Sunflower-Mildew Interaction
Plant Cell Physiol., March 1, 2009; 50(3): 665 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Chaki, A. M. Fernandez-Ocana, R. Valderrama, A. Carreras, F. J. Esteban, F. Luque, M. V. Gomez-Rodriguez, J. C. Begara-Morales, F. J. Corpas, and J. B. Barroso
Involvement of Reactive Nitrogen and Oxygen Species (RNS and ROS) in Sunflower-Mildew Interaction
Plant Cell Physiol., February 1, 2009; 50(2): 265 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. J. Corpas, M. Chaki, A. Fernandez-Ocana, R. Valderrama, J. M. Palma, A. Carreras, J. C. Begara-Morales, M. Airaki, L. A del Rio, and J. B. Barroso
Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions
Plant Cell Physiol., November 1, 2008; 49(11): 1711 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
U. Lee, C. Wie, B. O. Fernandez, M. Feelisch, and E. Vierling
Modulation of Nitrosative Stress by S-Nitrosoglutathione Reductase Is Critical for Thermotolerance and Plant Growth in Arabidopsis
PLANT CELL, March 1, 2008; 20(3): 786 - 802.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Neill, J. Bright, R. Desikan, J. Hancock, J. Harrison, and I. Wilson
Nitric oxide evolution and perception
J. Exp. Bot., January 1, 2008; 59(1): 25 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Jasid, M. Simontacchi, C. G. Bartoli, and S. Puntarulo
Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins
Plant Physiology, November 1, 2006; 142(3): 1246 - 1255.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.