JXB Advance Access originally published online on May 23, 2006
Journal of Experimental Botany 2006 57(9):2049-2059; doi:10.1093/jxb/erj158
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Society for Experimental Biology are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.orgThis paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
RESEARCH PAPER |
Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves
1Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University, N21W10, Kita-ku, Sapporo, 001-0021 Japan
2Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo, 060-8589 Japan
3National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
4Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854 Japan
5Hitachi Software Engineering, 4-12-7, Higashi-Shinagawa, Shinagawa, Tokyo, 140-0002 Japan
*To whom correspondence should be addressed. E-mail: junw{at}chem.agr.hokudai.ac.jp
Plants have developed several strategies for coping with phosphorus (P) deficiency. However, the details of the regulation of gene expression of adaptations to low P are still unclear. Using a cDNA microarray, transcriptomic analyses were carried out of the rice genes regulated by P deficiency and P re-supply to P-deficient plants. The OsPI1 gene, which was isolated as the most significant up-regulated gene under –P conditions, was also the most significant down-regulated gene following P re-supply. Many starch metabolism-related genes, as well as several genes for Pi-liberating enzymes, were up-regulated by –P treatment, suggesting a homeostatic contribution to the Pi concentration in leaf tissues. mRNAs for glucanases were also induced by P re-supply: these are suspected to play a role in loosening the cell wall compounds. Most of the genes up-regulated by –P treatment were down-regulated by P re-supply, suggesting that their responses were specific to –P conditions. Conversely, the number of genes up-regulated by P re-supply was also larger following P re-supply than in the –P condition. It is proposed that the genes up-regulated by P re-supply play an important role in P acquisition by P-deficient plants.
Key words: cDNA microarray, phosphorus deficiency, phosphorus re-supply, rice, transcriptome
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