Skip Navigation


JXB Advance Access originally published online on May 26, 2007
Journal of Experimental Botany 2007 58(10):2503-2512; doi:10.1093/jxb/erm082
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
58/10/2503    most recent
erm082v2
erm082v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Minic, Z.
Right arrow Articles by Jouanin, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minic, Z.
Right arrow Articles by Jouanin, L.
Agricola
Right arrow Articles by Minic, Z.
Right arrow Articles by Jouanin, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases

Zoran Minic1, Elisabeth Jamet2, Luc Négroni3, P Arsene der Garabedian4, Michel Zivy3 and Lise Jouanin1,*

1Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026 Versailles Cedex, France
2Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, 24 Chemin de Borde Rouge, BP42617, F-31326-Castanet-Tolosan, France
3Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université Paris XI, Institut National Agronomique Paris-Grignon, la Ferme du Moulon, F-91190 Gif-sur-Yvette, France
4Laboratoire d'Enzymologie des Acides Nucléiques, Université Pierre et Marie Curie, 96 Boulevard Raspail, F-75006 Paris, France

* To whom correspondence should be addressed. E-mail: jouanin{at}versailles.inra.fr

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using nanoHPLC-MS/MS and MALDI-TOF MS. 102 glycoproteins were identified. 94% of these proteins were predicted by bioinformatics to be targeted to the secretory pathway and 87% of them were predicted to be localized in the cell wall or at the plasma membrane. 30% of these proteins belong to glycoside hydrolase (GH) families with some of them possibly involved in the hydrolysis of cell wall polysaccharides. The second major class of identified proteins comprises aspartyl and serine proteases. Other proteins are predicted to be oxido-reductases, contain interacting domains, are potentially involved in signalling or have an unknown function. This is, to our knowledge, the first survey of plant cell wall N-glycosylated proteins.

Key words: Arabidopsis thaliana, cell wall, Concanavalin A, glycoside hydrolase, glycosylation, plant stem

Received 24 January 2007; Revised 21 March 2007 Accepted 21 March 2007


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
A. Eudes, G. Mouille, J. Thevenin, A. Goyallon, Z. Minic, and L. Jouanin
Purification, Cloning and Functional Characterization of an Endogenous Beta-glucuronidase in Arabidopsis Thaliana
Plant Cell Physiol., September 1, 2008; 49(9): 1331 - 1341.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Chavez Montes, P. Ranocha, Y. Martinez, Z. Minic, L. Jouanin, M. Marquis, L. Saulnier, L. M. Fulton, C. S. Cobbett, F. Bitton, et al.
Cell Wall Modifications in Arabidopsis Plants with Altered {alpha}-L-Arabinofuranosidase Activity
Plant Physiology, May 1, 2008; 147(1): 63 - 77.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.