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JXB Advance Access originally published online on June 18, 2007
Journal of Experimental Botany 2007 58(10):2525-2535; doi:10.1093/jxb/erm122
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Immunomodulation of jasmonate to manipulate the wound response

Petra ten Hoopen1 *, Astrid Hunger2, Axel Müller3, Bettina Hause2, Robert Kramell2, Claus Wasternack2, Sabine Rosahl2 and Udo Conrad1,{dagger}

1Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben, Corrensstrasse 3, D-06466 Gatersleben, Germany
2Leibniz-Institut für Pflanzenbiochemie Halle, Weinberg 3, D-06120 Halle (Saale), Germany
3Lehrstuhl für Pflanzenphysiologie der Ruhruniversität Bochum, Universitätsstraße 150, D-44801 Bochum, Germany

{dagger} To whom correspondence should be addressed. E-mail: conradu{at}IPK-gatersleben.de

Jasmonates are signals in plant stress responses and development. The exact mode of their action is still controversial. To modulate jasmonate levels intracellularly as well as compartment-specifically, transgenic Nicotiana tabacum plants expressing single-chain antibodies selected against the naturally occurring (3R,7R)-enantiomer of jasmonic acid (JA) were created in the cytosol and the endoplasmic reticulum. Consequently, the expression of anti-JA antibodies in planta caused JA-deficient phenotypes such as insensitivity of germinating transgenic seedlings towards methyl jasmonate and the loss of wound-induced gene expression. Results presented here suggest an essential role for cytosolic JA in the wound response of tobacco plants. The findings support the view that substrate availability takes part in regulating JA biosynthesis upon wounding. Moreover, high JA levels observed in immunomodulated plants in response to wounding suggest that tobacco plants are able to perceive a reduced level of physiologically active JA and attempt to compensate for this by increased JA accumulation.

Key words: Expression analysis, immunomodulation, jasmonate, wound response

* Present address: Department of Developmental Genetics/Section Plant Physiology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

Received 18 January 2007; Accepted 2 May 2007


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