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JXB Advance Access originally published online on June 19, 2007
Journal of Experimental Botany 2007 58(10):2641-2652; doi:10.1093/jxb/erm113
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Molecular characterization and expression analysis of the Rop GTPase family in Vitis vinifera

Philippe Abbal1, Martine Pradal1, François-Xavier Sauvage1, Philippe Chatelet2, Sophie Paillard3, Aurélie Canaguier3, Anne-Françoise Adam-Blondon3 and Catherine Tesniere1,*

1UMR1083, Sciences pour l’OEnologie, AgroM, INRA, Université Montpellier 1, F-34000 Montpellier, France
2UMR1098, Biologie du développement des Espèces Pérennes Cultivées, INRA, F-34060 Montpellier, France
3UMR1165, Génomique Végétale, INRA, F-91057 Evry, France

* To whom correspondence should be addressed. E-mail: tesniere{at}supagro.inra.fr

Rop/Rac GTPases are plant-specific signalling proteins with multiple roles, some of which have implications in plant development and in hormone signal transduction. Using expressed sequence tag (EST) and gene database analyses, members of the Rop family were characterized for the first time in a perennial species (Vitis vinifera). The grapevine genome was found to contain seven expressed VvRops. The phylogenetic analyses indicated that VvRops could be distributed into four groups, as described in the literature for model plants. Genetic mapping was successfully performed for five VvRops, which were localized on independent linkage groups. Conserved and divergent regions were identified on the protein sequences. The results of VvRop expression obtained by real-time quantitative reverse transcription-PCR analyses indicated that all the organs investigated displayed VvRop expression, however with different patterns. Whereas no total organ specificity for VvRop expression could be evidenced, VvRop9 displayed high expression in developing berries only. During berry development, the transcript profile was generally similar for all the VvRops, i.e. displaying a peak early in the herbaceous phase followed by a decline towards veraison and thereafter. Western blotting gave a similar expression profile for VvRop proteins. Response to growth regulators was generally specific to each VvRop. The potential involvement of specific VvRops in grapevine development is discussed.

Key words: EST database, fruit ripening, genome mapping, grapevine phylogeny, quantitative RT-PCR, Rop/Rac GTPase, transcript level, Vitis vinifera

Received 13 March 2007; Revised 23 April 2007 Accepted 25 April 2007


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P. Abbal, M. Pradal, L. Muniz, F.-X. Sauvage, P. Chatelet, T. Ueda, and C. Tesniere
Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein
J. Exp. Bot., June 1, 2008; 59(9): 2403 - 2416.
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