JXB Advance Access originally published online on August 28, 2007
Journal of Experimental Botany 2007 58(12):3155-3169; doi:10.1093/jxb/erm153
© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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RESEARCH PAPER |
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
1CSIRO Plant Industry, Canberra, ACT 2601, Australia
2Department of Rice Research, National Agricultural Research Center, Joetsu, Niigata, 943-0193 Japan
To whom correspondence should be addressed. E-mail: robert.furbank{at}csiro.au
The roles of the rice sucrose transporter, OsSUT1, have previously been examined in filling grain, germination, and early seedling growth. In the current work, the role that OsSUT1 plays in the transport of assimilate along the entire long-distance pathway, from the flag leaf blade to the base of the filling grain, was investigated. OsSUT1 promoter::GUS (β-glucuronidase) reporter gene analysis and immunolocalization revealed that both OsSUT1 promoter::GUS activity and OsSUT protein were present in the mature phloem of all the vegetative tissues involved in the long-distance assimilate transport pathway during grain filling. In addition, expression was observed in the flag leaf blade and sheath prior to heading. The OsSUT1 promoter::GUS activity appeared to be largely confined to the companion cells within the phloem, whereas the protein localized to both the sieve tubes and the companion cells. RT-PCR analysis confirmed that the OsSUT1 transcript is expressed in the uppermost internode of the rice plant (internode-1). These OsSUT localization data were related to measurements of starch and soluble sugar content of these tissues, and localization of the carbohydrate reserves stored in the stem. Results from dye feeding experiments, to examine cellular connections, revealed a symplastic continuity between the phloem and surrounding parenchyma in the flag leaf blade, sheath, and internode-1 tissues. It is proposed that OsSUT1 may primarily play a role in phloem loading of sucrose retrieved from the apoplasm along the transport pathway.
Key words: Long-distance pathway, OsSUT1, rice, starch remobilization, sucrose transport, transport phloem
* Present address: Laboratory of Crop Science Graduate School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunko, Tokyo 113-8657, Japan.
Received 1 May 2007; Revised 6 June 2007 Accepted 11 June 2007
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