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Journal of Experimental Botany 2007 58(15-16):4147-4159; doi:10.1093/jxb/erm272
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana

Ming-Yan Cheung1, Nai-Yan Zeng1, Suk-Wah Tong1, Francisca Wing-Yen Li1, Kai-Jun Zhao2, Qi Zhang2, Samuel Sai-Ming Sun1,3 and Hon-Ming Lam1,3,*

1Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR
2Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, PRC
3Molecular Biotechnology Program, The Chinese University of Hong Kong, Shatin, Hong Kong SAR

* To whom correspondence should be addressed. E-mail: honming{at}cuhk.edu.hk

A cDNA clone (OsRHC1) was obtained, which encodes a novel RING zinc finger protein sharing similar structural features (multiple transmembrane domains at the N-half; a unique RING zinc finger consensus Cys-X2-Cys-X11-Cys-X-His-X3-Cys-X2-Cys-X6-Cys-X2-Cys at the C terminus) to a group of closely related annotated proteins from both monocots and dicots. OsRHC1 was found to be localized on plasma membrane of rice cells and induced by wounding in rice lines containing Xa loci. Ecotopic expression of the OsRHC1 cDNA from rice (a monocot) in transgenic Arabidopsis thaliana (a dicot) enhanced the defence response toward Pseudomonas syringae pv. tomato DC3000, suggesting that OsRHC1 may confer broad-spectrum disease resistance. The protective effects were neutralized in the presence of MG132 or in an npr1-3 mutation background, indicating that the function of OsRHC1 is dependent on the ubiquitin-mediated protein degradation via the 26S proteasome and the presence of the key defence response regulator NPR1.

Key words: E3 ubiquitin ligase, plant disease resistance, RING zinc finger protein, signal transduction

Received 30 May 2007; Revised 18 September 2007 Accepted 9 October 2007


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