JXB Advance Access originally published online on November 16, 2006
Journal of Experimental Botany 2007 58(2):229-240; doi:10.1093/jxb/erl163
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RESEARCH PAPER |
Barley transcript profiles under dehydration shock and drought stress treatments: a comparative analysis
1Department of Agroenvironmental Sciences and Technology, University of Bologna, 40127 Bologna, Italy
2Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA
3Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
To whom correspondence should be addresssed. E-mail: roberto.tuberosa{at}unibo.it
A microarray including 1654 cDNAs, mainly derived from dehydration-shocked barley leaf tissues, was utilized to monitor expression changes in leaves of barley plants subjected to slow drying conditions (7 d and 11 d: 7d-WS and 11d-WS) in soil and after rehydration. The results were compared with those obtained under shock-like conditions imposed with a 6 h dehydration treatment. A total number of 173 transcripts (
10% of all transcripts profiled) were declared up- or down-regulated in at least one of the conditions tested. The majority of the transcripts were regulated by only one of the drought treatments, with 57% of the differentially expressed transcripts exclusively affected in the dehydration shock treatment, 6% at 7d-WS, 14% at 11d-WS, and 6% after rehydration. Irrespective of the low percentage of transcripts (10%) with similar expression changes between shock and slow stress treatments, a sizeable portion of these transcripts shared a common expression trend under the different drought treatment conditions, as evidenced by low but significant correlations between the fast occurring and the 7d-WS and 11d-WS treatments (r=0.32 and 0.41, P=0.001, respectively). These results are discussed with respect to the merit of different dehydration treatments in the investigation of the changes in transcript profiling.
Key words: Barley, cDNA microarray, dehydration shock, drought stress, drought-responsive transcripts
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