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Journal of Experimental Botany 2007 58(2):241-252; doi:10.1093/jxb/erl262
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Early PLD{alpha}-mediated events in response to progressive drought stress in Arabidopsis: a transcriptome analysis

Shrinivasrao P. Mane1, Cecilia Vasquez-Robinet1, Allan A. Sioson2 *, Lenwood S. Heath2 and Ruth Grene1,{dagger}

1Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA
2Department of Computer Science, Virginia Tech, Blacksburg, VA 24061, USA

{dagger} To whom correspondence should be addressed. E-mail: grene{at}vt.edu

Phospholipase D (PLD) has been implicated in a variety of stresses including osmotic stress and wounding. PLD{alpha}1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and promotes abscisic acid signalling. It has also been shown to regulate proline biosynthesis negatively. Plants with abrogated PLD{alpha} show insensitivity to abscisic acid (ABA) and impaired stomatal conductance. The goal in the present study was to identify early PLD{alpha}-mediated events in response to progressive drought stress in Arabidopsis. Water was withheld from 7-week-old Arabidopsis thaliana (Col-0) and antisense-PLD{alpha}1 (anti-PLD{alpha}) in a controlled environment chamber. Diurnal leaf water potential (LWP) and photosynthesis measurements were recorded five and three times a day, respectively. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) and microarray analyses were conducted using RNA from shoots collected at the fourth LWP time point on the ninth day after stress imposition. Anti-PLD{alpha} experienced severe water stress (–1.28 MPa) at the same time period that Col-0 experienced less water stress (–0.31 MPa). Diurnal LWP measurements showed that anti-PLD{alpha} had a lower LWP than Col-0 in both control and drought-stress conditions. Photosynthesis was also more affected in anti-PLD{alpha} than in Col-0. Anti-PLD{alpha} plants recovered fully following rehydration after 10 d of stress. qRT–PCR revealed up to 18-fold lower values for PLD{alpha} transcripts in stressed anti-PLD{alpha} plants when compared with stressed Col-0. Microarray expression profiles revealed distinct gene expression patterns in Col-0 and anti-PLD{alpha}. No differences in gene expression were detected between the two genotypes in the absence of drought stress. ROP8, PLD{delta}, and lipid transfer proteins were among the differentially expressed genes between the two genotypes.


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