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JXB Advance Access originally published online on January 8, 2007
Journal of Experimental Botany 2007 58(3):507-520; doi:10.1093/jxb/erl258
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Identification of early salt stress response genes in tomato root by suppression subtractive hybridization and microarray analysis

Bo Ouyang1,2, Ting Yang1, Hanxia Li2, Liang Zhang3, Yuyang Zhang1, Junhong Zhang1, Zhangjun Fei4 *,{dagger} and Zhibiao Ye1,{dagger}

1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
2Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
3National Engineering Research Center for Beijing Biochip Technology, Changping District, Beijing 102206, China
4Virginia Bioinformatics Institute, Virginia Tech University, Blacksburg, VA 24061, USA

{dagger} To whom correspondence should be addressed. E-mail: zbye{at}mail.hzau.edu.cn and zfei{at}vt.edu

High salinity is one of the most serious threats to crop production. To understand the molecular basis of plant responses to salt stress better, suppression subtractive hybridization (SSH) and microarray approaches were combined to identify the potential important or novel genes involved in the early stage of tomato responses to severe salt stress. First, SSH libraries were constructed for the root tissue of two cultivated tomato (Solanum lycopersicum) genotypes: LA2711, a salt-tolerant cultivar, and ZS-5, a salt-sensitive cultivar, to compare salt treatment and non-treatment plants. Then a subset of clones from these SSH libraries were used to construct a tomato cDNA array and microarray analysis was carried out to verify the expression changes of this set of clones upon a high concentration of salt treatment at various time points compared to the corresponding non-treatment controls. A total of 201 non-redundant genes that were differentially expressed upon 30 min of severe salt stress either in LA2711 or ZS-5 were identified from microarray analysis; most of these genes have not previously been reported to be associated with salt stress. The diversity of the putative functions of these genes indicated that salt stress resulted in a complex response in tomato plants.

Key words: Gene expression, genotype, microarray, salt stress, SSH, tomato

* Present address: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853 and USDA Plant, Soil, and Nutrition Laboratory, Tower Rd, Ithaca, NY 14853, USA.

Received 18 May 2006; Revised 17 September 2006 Accepted 26 October 2006


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