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JXB Advance Access originally published online on December 22, 2006
Journal of Experimental Botany 2007 58(4):773-784; doi:10.1093/jxb/erl257
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Leaf Scale Studies: Combination Imaging and Stress Diagnosis

Monitoring and screening plant populations with combined thermal and chlorophyll fluorescence imaging

Laury Chaerle1,*, Ilkka Leinonen2, Hamlyn G. Jones2 and Dominique Van Der Straeten1,*

1Unit of Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
2Plant Research Unit, Division of Environmental and Applied Biology, University of Dundee at SCRI, Invergowrie, Dundee DD2 5DA, UK

* To whom correspondence should be addressed. E-mail: laury.chaerle{at}ugent.be; dominique.vanderstraeten{at}ugent.be

Thermal and chlorophyll fluorescence imaging are powerful tools for the study of spatial and temporal heterogeneity of leaf transpiration and photosynthetic performance. The relative advantages and disadvantages of these techniques are discussed. When combined, they can highlight pre-symptomatic responses not yet apparent in visual spectrum images and provide specific signatures for diagnosis of distinct diseases and abiotic stresses. In addition, their use for diagnosis and for selection for stomatal or photosynthetic mutants, these techniques can be applied for stress tolerance screening. For example, rapid screening for stomatal responses can be achieved by thermal imaging, while, combined with fluorescence imaging to study photosynthesis, they can potentially be used to derive leaf water use efficiency as a screening parameter. A particular advantage of imaging is that it allows continuous automated monitoring of dynamic spatial variation. Examples of applications include the study of growth and development of plant lines differing in stress resistance, yield, circadian clock-controlled responses, and the possible interactions between these parameters. In the future, such dual-imaging systems could be extended with complementary techniques such as hyperspectral and blue-green fluorescence imaging. This would result in an increased number of quantified parameters which will increase the power of stress diagnosis and the potential for screening of stress-tolerant genotypes.

Key words: Chlorophyll fluorescence imaging, hypersensitive reaction, rhythm, screening, stress, thermography

Received 8 May 2006; Revised 19 October 2006 Accepted 24 October 2006


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