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JXB Advance Access originally published online on February 23, 2007
Journal of Experimental Botany 2007 58(6):1381-1396; doi:10.1093/jxb/erl304
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Receptor-like protein kinase HvLysMR1 of barley (Hordeum vulgare L.) is induced during leaf senescence and heavy metal stress

Akli Ouelhadj1, Marc Kaminski2, Maria Mittag2 and Klaus Humbeck1,*

1Martin-Luther Universität Halle-Wittenberg, Institut für Biologie, Weinbergweg 10, D-06120 Halle, Germany
2Friedrich-Schiller-Universität Jena, Institut für Allgemeine Botanik, Am Planetarium 1, D-07743 Jena, Germany

* To whom correspondence should be addressed. E-mail: klaus.humbeck{at}pflanzenphys.uni-halle.de

The Hordeum vulgare cDNA clone HvLysMR1 that encodes a putative receptor-like protein kinase was identified by restriction fragment differential display–polymerase chain reaction (PCR) comparing cDNA populations derived from mRNAs of primary leaves stressed with chromium for 48 h with controls. The full-length sequence codes for a protein with 622 amino acids which includes characteristic domains of lysine motif receptor-like kinases: an N-terminal signal peptide, two lysine motifs, a transmembrane region, and a serine/threonine kinase domain at the C-terminal end. The expression of HvLysMR1 is induced during exposure to different heavy metals and its transcript accumulates during leaf senescence. Addition of the calcium ionophore A23187 [GenBank] induces HvLysMR1 expression, indicating the involvement of Ca2+ in the regulation of HvLysMR1. In vitro phosphorylation of HvLysMR1 was analysed with [32P]ATP. Using the overexpressed and purified HvLysMR1–kinase domain, the phosphorylation of HvLysMR1 could be confirmed by nano-liquid chromatography-electrospray ionization-mass spectrometry (LC–ESI–MS) with neutral loss-triggered MS–MS–MS spectra at amino acids localized at the juxtamembrane region. The involvement of HvLysMR1 during heavy metal stress and leaf senescence is discussed.

Key words: Calcium, heavy metal stress, Hordeum vulgare, leaf senescence, lysine motif, receptor-like kinase

Received 19 September 2006; Revised 12 December 2006 Accepted 18 December 2006


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