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JXB Advance Access originally published online on February 21, 2007
Journal of Experimental Botany 2007 58(6):1407-1420; doi:10.1093/jxb/erm004
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Engineering of gibberellin levels in citrus by sense and antisense overexpression of a GA 20-oxidase gene modifies plant architecture

Carmen Fagoaga1, Francisco R. Tadeo3, Domingo J. Iglesias3, Laura Huerta2, Ignacio Lliso3, Ana M. Vidal2, Manuel Talon3, Luís Navarro1, José L. García-Martínez2 and Leandro Peña1,*

1Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, Moncada 46113, Valencia, Spain
2Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, Avenida de los Naranjos s/n, Valencia 46022, Spain
3Centro de Genómica, Instituto Valenciano de Investigaciones Agrarias, (IVIA), Apdo. Oficial, Moncada 46113, Valencia, Spain

* To whom correspondence should be addressed. E-mail: lpenya{at}ivia.es

Carrizo citrange (Citrus sinensisxPoncirus trifoliata) is a citrus hybrid widely used as a rootstock, whose genetic manipulation to improve different growth characteristics is of high agronomic interest. In this work, transgenic Carrizo citrange plants have been produced overexpressing sense and antisense CcGA20ox1 (a key enzyme of GA biosynthesis) under control of the 35S promoter to modify plant architecture. As expected, taller (sense) and shorter (antisense) phenotypes correlated with higher and lower levels, respectively, of active GA1 in growing shoots. In contrast, other phenotypic characteristics seemed to be specific to citrus, or different from those described for similar transgenics in other species. For instance, thorns, typical organs of citrus at juvenile stages, were much longer in sense and shorter in antisense plants, and xylem tissue was reduced in leaf and internode of sense plants. Antisense plants presented a bushy phenotype, suggesting a possible effect of GAs on auxin biosynthesis and/or transport. The main foliole of sense plants was longer, although total leaf area was reduced. Leaf thickness was smaller in sense and larger in antisense plants due to changes in the spongy parenchyma. Internode cell length was not altered in transgenic plants, indicating that, in citrus, GAs regulate cell division rather than cell elongation. Interestingly, the phenotypes described were not apparent when transgenic plants were grafted on non-transgenic rootstock. This suggests that roots contribute to the GA economy of aerial parts in citrus and opens the possibility of using the antisense plants as dwarfing rootstocks.

Key words: Carrizo citrange, dwarfing, gibberellin 20-oxidase, transgenic plants

Received 16 November 2006; Revised 19 December 2006 Accepted 5 January 2007


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 Tree PhysiolHome page
L. Huerta, A. Garcia-Lor, and J. L. Garcia-Martinez
Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange
Tree Physiol, April 1, 2009; 29(4): 569 - 577.
[Abstract] [Full Text] [PDF]


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