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JXB Advance Access originally published online on February 24, 2007
Journal of Experimental Botany 2007 58(6):1463-1472; doi:10.1093/jxb/erm008
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Elicitor and resistance-inducing activities of ß-1,4 cellodextrins in grapevine, comparison with ß-1,3 glucans and {alpha}-1,4 oligogalacturonides

Aziz Aziz1,*, Adrien Gauthier2, Annie Bézier1, Benoît Poinssot2, Jean-Marie Joubert3, Alain Pugin2, Alain Heyraud4 and Fabienne Baillieul1

1URVVC-EA 2069, Stress, Défenses et Reproduction des Plantes, Université de Reims Champagne-Ardenne, BP 1039, F-51687 Reims cedex 2, France
2UMR Plante–Microbe–Environnement, INRA 1088, CNRS 5184, Université de Bourgogne, 17 rue Sully, BP 86510, 21065 Dijon cedex, France
3Laboratoires Goëmar, Avenue du Général Patton, F-35400 Saint Malo cedex, France
4Centre de Recherche sur les Macromolécules Végétales, CNRS, BP 53 X, F-38041 Grenoble, France

* Present address and to whom correspondence should be sent: Plantes, Pesticides, Développement Durable, URVVC-EA 2069, Université de Reims, BP 1039, F-51687 Reims cedex 2, France. E-mail: aziz.aziz{at}univ-reims.fr

Cellodextrins (CD), water-soluble derivatives of cellulose composed of ß-1,4 glucoside residues, have been shown to induce a variety of defence responses in grapevine (Vitis vinifera L.) cells. The larger oligomers of CD rapidly induced transient generation of H2O2 and elevation in free cytosolic calcium, followed by a differential expression of genes encoding key enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins as well as stimulation of chitinase and ß-1,3 glucanase activities. Most of these defence reactions were also induced by linear ß-1,3 glucans (ßGlu) and {alpha}-1,4 oligogalacturonides (OGA) of different degree of polymerization (DP), but the intensity of some reactions induced by CD was different when compared with ßGlu and OGA effects. Moreover, desensitization assays using H2O2 production showed that cells treated with CD remained fully responsive to a second application of OGA, suggesting a different mode of perception of these oligosaccharides by grape cells. None of CD, ßGlu, or OGA induced HSR gene expression nor did they induce cell death. In accordance with elicitor activity in grapevine cells, CD-incubated leaves challenged with Botrytis cinerea also resulted in a significant reduction of the disease. Data suggest that CD could operate via other distinct reaction pathways than ßGlu and OGA. They also highlight the requirement of a specific DP for each oligosaccharide to induce the defence response.

Key words: Cellodextrins, defence responses, grapevine, induced resistance

Received 20 September 2006; Revised 18 December 2006 Accepted 5 January 2007


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