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JXB Advance Access originally published online on April 2, 2007
Journal of Experimental Botany 2007 58(7):1753-1760; doi:10.1093/jxb/erm030
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Can dual chlorophyll fluorescence excitation be used to assess the variation in the content of UV-absorbing phenolic compounds in leaves of temperate tree species along a light gradient?

Sandrine Barthod1, Zoran Cerovic2 and Daniel Epron3,*

1Université de Franche-Comté, Laboratoire de Biologie Environnementale, Pôle de Montbéliard, 4 place Tharradin, BP 71427, F-25 211 Montbéliard Cedex, France
2Equipe de Biospectroscopie Végétale, Département d' Écophysiologie Végétale, Laboratoire d'Ecologie Systémique et Evolution, Université de Paris Sud, Bâtiment 362, F-91405 Orsay Cedex, France
3Nancy University, Université Henri Poincaré, UMR 1137 INRA–UHP Ecologie et Ecophysiologie Forestières, BP 239, F-54506 Vandoeuvre Cedex, France

* To whom correspondence should be addressed. E-mail: daniel.epron{at}scbiol.uhp-nancy.fr

The present study assesses light-induced variations in phenolic compounds in leaves of saplings of two co-occurring temperate species (Acer platanoides L., and Fraxinus excelsior L.) along a light gradient using a new non-invasive optical method (Dualex). The Dualex-derived UV absorbance of leaf epidermis (the sum of the adaxial and abaxial faces, AUV) increased significantly with increasing light in both species. AUV values were correlated with absorbance of the leaf extract at 305 nm and 375 nm (A305 and A375) in both species with similar slopes for both species. However, a large difference in intercept was observed between the two species when A305 was regressed against AUV. Similarly, AUV values were well correlated with the amount of phenolics in the leaf extracts assessed by the Folin–Ciocalteu method, but slopes were significantly different for the two species. Thus, the UV-A epidermal transmittance, despite being a reliable indicator of the UV-screening capacity of the leaf epidermis, cannot be used for any quantitative estimate of UV-B screening capacity or of energetic requirement for leaf construction without a species-specific calibration.

Key words: Acer platanoides L., epidermis, fluorescence, Fraxinus excelsior L., phenolic compounds, UV absorbance, shade

Received 22 September 2006; Revised 20 January 2007 Accepted 30 January 2007


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