JXB Advance Access originally published online on March 23, 2007
Journal of Experimental Botany 2007 58(7):1795-1802; doi:10.1093/jxb/erm037
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
RESEARCH PAPER |
Organization of pectic arabinan and galactan side chains in association with cellulose microfibrils in primary cell walls and related models envisaged
UR 1268 Biopolymères, Interactions, Assemblages, INRA, F-44300 Nantes, France
* To whom correspondence should be addressed. E-mail: ralet{at}nantes.inra.fr
The structure of arabinan and galactan domains in association with cellulose microfibrils was investigated using enzymatic and alkali degradation procedures. Sugar beet and potato cell wall residues (called ‘natural’ composites), rich in pectic neutral sugar side chains and cellulose, as well as ‘artificial’ composites, created by in vitro adsorption of arabinan and galactan side chains onto primary cell wall cellulose, were studied. These composites were sequentially treated with enzymes specific for pectic side chains and hot alkali. The degradation approach used showed that most of the arabinan and galactan side chains are in strong interaction with cellulose and are not hydrolysed by pectic side chain-degrading enzymes. It seems unlikely that isolated arabinan and galactan chains are able to tether adjacent microfibrils. However, cellulose microfibrils may be tethered by different pectic side chains belonging to the same pectic macromolecule.
Key words:
Arabinan, galactan, enzymes, 
-L-arabinofuranosidase, endo-arabinanase, ß-galactosidase, endo-galactanase
Received 6 December 2006; Revised 8 February 2007 Accepted 8 February 2007
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