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JXB Advance Access originally published online on April 2, 2007
Journal of Experimental Botany 2007 58(7):1825-1834; doi:10.1093/jxb/erm045
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Suppression subtractive hybridization identifies genes induced in response to UV-B irradiation in apple skin: isolation of a putative UDP-glucose 4-epimerase

Yusuke Ban1, Chikako Honda2, Hideo Bessho3, Xiao-Ming Pang2 * and Takaya Moriguchi1,2,{dagger}

1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan
2National Institute of Fruit Tree Science, Tsukuba, Ibaraki, 305-8605 Japan
3National Institute of Fruit Tree Science, Morioka, Iwate, 020-0123 Japan

{dagger} To whom correspondence should be addressed. E-mail: takaya{at}affrc.go.jp

Suppression subtractive hybridization (SSH) successfully identified 11 cDNAs in apple skin with highly induced expression as a result of ultraviolet (UV)-B irradiation. Apart from three putative flavonoid biosynthetic genes, chalcone synthase (CHS; A5C), flavanone-3-hydroxylase (F3H; B5F), and flavonol synthase (FLS; D1F), five clones (A1H, A10E, B11G, D5F, and D11H) were induced by low temperature (17 °C) as well, which is also known to induce anthocyanin accumulation in apple skin. Moreover, four clones (A1H, A10E, B11G, and D11H), showing higher expression levels in the skin, accumulated higher anthocyanin concentrations than their counterparts. Of the four clones, only A10E, a putative UDP-glucose 4-epimerase (UGE), was deemed to play an important role in anthocyanin accumulation in apple skin based on the facts that: (i) its transcription level was higher in the deep red cultivar, ‘Jonathan’, than in the pale red cultivar, ‘Tsugaru’; and (ii) it could reversibly catalyse UDP-glucose to UDP-galactose, and the latter molecule is a major sugar donor for cyanidin-glycoside in apple. Therefore, the full-length cDNA of A10E was isolated by rapid amplification of cDNA ends (RACE) and designated as MdUGE1. Further analysis demonstrated that UGE enzymatic activity was positively correlated with anthocyanin accumulation in apple skin. Thus, MdUGE1 isolated by SSH could play an important role in anthocyanin biosynthesis in apple skin in concert with other flavonoid biosynthetic genes.

Key words: Anthocyanin, apple (Malusxdomestica) skin, gene expression, suppression subtractive hybridization, UDP-glucose 4-epimerase (UGE), UV-B

* Present address: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, PR China.

Received 27 September 2006; Revised 18 February 2007 Accepted 19 February 2007


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This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. Ban, C. Honda, Y. Hatsuyama, M. Igarashi, H. Bessho, and T. Moriguchi
Isolation and Functional Analysis of a MYB Transcription Factor Gene that is a Key Regulator for the Development of Red Coloration in Apple Skin
Plant Cell Physiol., July 1, 2007; 48(7): 958 - 970.
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