Skip Navigation


JXB Advance Access originally published online on May 23, 2008
Journal of Experimental Botany 2008 59(10):2639-2647; doi:10.1093/jxb/ern118
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
59/10/2639    most recent
ern118v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Delmas, F.
Right arrow Articles by Chevalier, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delmas, F.
Right arrow Articles by Chevalier, C.
Agricola
Right arrow Articles by Delmas, F.
Right arrow Articles by Chevalier, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

The synthesis of the rhamnogalacturonan II component 3-deoxy-D-manno-2-octulosonic acid (Kdo) is required for pollen tube growth and elongation

Frédéric Delmas1,4, Martial Séveno3, Julian G. B. Northey4, Michel Hernould1,2, Patrice Lerouge3, Peter McCourt4 and Christian Chevalier1,2,*

1INRA (Institut National de la Recherche Agronomique), Unité Mixte de Recherche 619 sur la Biologie du Fruit, Institut Fédératif de Recherche 103, F-33883 Villenave d'Ornon, France
2Université Victor Segalen Bordeaux 2, Unité Mixte de Recherche 619 sur la Biologie du Fruit, Institut Fédératif de Recherche 103, F-33883 Villenave d'Ornon, France
3Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, Laboratoire des Transports Intracellulaires, IFRMP 23, Université de Rouen, F-76821 Mont Saint Aignan, France
4University of Toronto, Cell and Systems Biology Laboratory, 25 Willcocks Street, Toronto, Ontario M5S3B2, Canada

* To whom correspondence should be addressed. E-mail: chevalie{at}bordeaux.inra.fr

Despite a very complex structure, the sugar composition of the rhamnogalacturonan II (RG-II) pectic fraction is extremely conserved. Among its constituting monosaccharides is the seldom-observed eight-carbon sugar 3-deoxy-D-manno-octulosonic acid (Kdo), whose phosphorylated precursor is synthesized by Kdo-8-P synthase. As an attempt to alter specifically the RG-II structure in its sugar composition and assess the consequences on the function of RG-II in cell wall and its relationship with growth, Arabidopsis null mutants were sought in the genes encoding Kdo-8-P synthase. Here, the isolation and characterization of one null mutant for the isoform 1 (AtkdsA1-S) and two distinct null mutants for the isoform 2 of Arabidopsis Kdo-8-P synthase (AtkdsA2-V and AtkdsA2-S) are described. Evidence is provided that AtkdsA2 gene expression is preferentially associated with plantlet organs displaying a meristematic activity, and that it accounts for 75% of the mRNAs to be translated into Kdo-8-P synthase. Furthermore, this predominant expression of AtKDSA2 over AtKDSA1 was confirmed by quantification of the cytosolic Kdo content in the mutants, in a variety of ecotypes. The inability to identify a double knockout mutant originated from pollen abortions, due to the inability of haploid pollen of the AtkdsA1- AtkdsA2- genotype to form an elongated pollen tube properly and perform fertilization.

Key words: Arabidopsis thaliana, 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate, 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate synthase, pollen tube growth, rhamnogalacturonan II

Received 5 February 2008; Accepted 2 April 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.