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JXB Advance Access originally published online on May 23, 2008
Journal of Experimental Botany 2008 59(10):2639-2647; doi:10.1093/jxb/ern118
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

The synthesis of the rhamnogalacturonan II component 3-deoxy-D-manno-2-octulosonic acid (Kdo) is required for pollen tube growth and elongation

Frédéric Delmas1,4, Martial Séveno3, Julian G. B. Northey4, Michel Hernould1,2, Patrice Lerouge3, Peter McCourt4 and Christian Chevalier1,2,*

1INRA (Institut National de la Recherche Agronomique), Unité Mixte de Recherche 619 sur la Biologie du Fruit, Institut Fédératif de Recherche 103, F-33883 Villenave d'Ornon, France
2Université Victor Segalen Bordeaux 2, Unité Mixte de Recherche 619 sur la Biologie du Fruit, Institut Fédératif de Recherche 103, F-33883 Villenave d'Ornon, France
3Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, Laboratoire des Transports Intracellulaires, IFRMP 23, Université de Rouen, F-76821 Mont Saint Aignan, France
4University of Toronto, Cell and Systems Biology Laboratory, 25 Willcocks Street, Toronto, Ontario M5S3B2, Canada

* To whom correspondence should be addressed. E-mail: chevalie{at}bordeaux.inra.fr

Despite a very complex structure, the sugar composition of the rhamnogalacturonan II (RG-II) pectic fraction is extremely conserved. Among its constituting monosaccharides is the seldom-observed eight-carbon sugar 3-deoxy-D-manno-octulosonic acid (Kdo), whose phosphorylated precursor is synthesized by Kdo-8-P synthase. As an attempt to alter specifically the RG-II structure in its sugar composition and assess the consequences on the function of RG-II in cell wall and its relationship with growth, Arabidopsis null mutants were sought in the genes encoding Kdo-8-P synthase. Here, the isolation and characterization of one null mutant for the isoform 1 (AtkdsA1-S) and two distinct null mutants for the isoform 2 of Arabidopsis Kdo-8-P synthase (AtkdsA2-V and AtkdsA2-S) are described. Evidence is provided that AtkdsA2 gene expression is preferentially associated with plantlet organs displaying a meristematic activity, and that it accounts for 75% of the mRNAs to be translated into Kdo-8-P synthase. Furthermore, this predominant expression of AtKDSA2 over AtKDSA1 was confirmed by quantification of the cytosolic Kdo content in the mutants, in a variety of ecotypes. The inability to identify a double knockout mutant originated from pollen abortions, due to the inability of haploid pollen of the AtkdsA1- AtkdsA2- genotype to form an elongated pollen tube properly and perform fertilization.

Key words: Arabidopsis thaliana, 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate, 3-deoxy-D-manno-oct-2-ulosonate-8-phosphate synthase, pollen tube growth, rhamnogalacturonan II

Received 5 February 2008; Accepted 2 April 2008


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