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JXB Advance Access originally published online on May 29, 2008
Journal of Experimental Botany 2008 59(10):2717-2733; doi:10.1093/jxb/ern138
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Ethylene-induced differential gene expression during abscission of citrus leaves

Javier Agustí {dagger}, Paz Merelo, Manuel Cercós, Francisco R. Tadeo* and Manuel Talón

Instituto Valenciano de Investigaciones Agrarias, Centro de Genómica, Ctra. de Moncada-Náquera km 4.5, E-46113 Moncada, Valencia, Spain

* To whom correspondence should be addressed. E-mail: tadeo-fra{at}gva.es

The main objective of this work was to identify and classify genes involved in the process of leaf abscission in Clementina de Nules (Citrus clementina Hort. Ex Tan.). A 7 K unigene citrus cDNA microarray containing 12 K spots was used to characterize the transcriptome of the ethylene-induced abscission process in laminar abscission zone-enriched tissues and the petiole of debladed leaf explants. In these conditions, ethylene induced 100% leaf explant abscission in 72 h while, in air-treated samples, the abscission period started later and took 240 h. Gene expression monitored during the first 36 h of ethylene treatment showed that out of the 12 672 cDNA microarray probes, ethylene differentially induced 725 probes distributed as follows: 216 (29.8%) probes in the laminar abscission zone and 509 (70.2%) in the petiole. Functional MIPS classification and manual annotation of differentially expressed genes highlighted key processes regulating the activation and progress of the cell separation that brings about abscission. These included cell-wall modification, lipid transport, protein biosynthesis and degradation, and differential activation of signal transduction and transcription control pathways. Expression data associated with the petiole indicated the occurrence of a double defensive strategy mediated by the activation of a biochemical programme including scavenging ROS, defence and PR genes, and a physical response mostly based on lignin biosynthesis and deposition. This work identifies new genes probably involved in the onset and development of the leaf abscission process and suggests a different but co-ordinated and complementary role for the laminar abscission zone and the petiole during the process of abscission.

Key words: Citrus clementina, cDNA microarray, expression profiling, laminar abscission zone, mandarin, petiole

{dagger} Present address: Gregor Mendel Institute for Plant Molecular Biology, Vienna Biocenter, Dr. Bohr Gasse 3, 1030 Wien, Austria.

Received 22 January 2008; Revised 9 April 2008 Accepted 9 April 2008


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