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JXB Advance Access originally published online on July 4, 2008
Journal of Experimental Botany 2008 59(11):3069-3076; doi:10.1093/jxb/ern161
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis

Naoto Mitsuhashi1,2 *, Maki Kondo3, Satoru Nakaune4, Miwa Ohnishi1,5, Makoto Hayashi3, Ikuko Hara-Nishimura4, Alan Richardson6, Hidehiro Fukaki1, Mikio Nishimura3 and Tetsuro Mimura1,5,{dagger}

1Department of Biology, Graduate School of Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501 Japan
2Japan Society for the Promotion of Science (JSPS), Chiyoda-ku, Tokyo, 102-8471 Japan
3Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan
4Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
5Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chuou-ku, Tokyo, 113-0027 Japan
6CSIRO Plant Industry, PO Box 1600, Canberra, ACT, 2601, Australia

{dagger} To whom correspondence should be adressed. E-mail: mimura{at}kobe-u.ac.jp

Expression and localization of myo-inositol-1-phosphate synthase (MIPS) in developing seeds of Arabidopsis thaliana was investigated. MIPS is an essential enzyme for production of inositol and inositol phosphates via its circularization of glucose-6-phosphate as the initial step. myo-inositol-6-phosphate (InsP6 or phytic acid) is the predominant form of phosphorus found in seeds and accumulates as a consequence of MIPS action. Three MIPS genes have been identified in Arabidopsis, all of which were expressed not only in siliques but in both leaves and roots. Immunoelectron microscopy using a MIPS antibody showed that MIPS localizes to the cytosol primarily in the endosperm during seed development and not in the embryo. This is consistent with results obtained using fluorescent microscopy and western blot analysis that showed a similar pattern of localization. However, InsP6, which is the final product of inositol phosphate metabolism, was present mainly in the embryo. This suggests that a complex interaction between the endosperm and embryo occurs during the synthesis and subsequent accumulation of InsP6 in developing seeds of Arabidopsis.

Key words: Inositol, inositol phosphates, inositol-6-phosphates, myo-inositol-1-phosphate synthase, phosphate, phytate, vacuole

* Present address: Protenova Co. Ltd, Hayashi-machi 2217-44, Takamatsu, Kagawa, 761-0301 Japan

Received 15 February 2008; Revised 7 May 2008 Accepted 12 May 2008


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