Skip Navigation


JXB Advance Access originally published online on July 8, 2008
Journal of Experimental Botany 2008 59(11):3121-3129; doi:10.1093/jxb/ern166
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
59/11/3121    most recent
ern166v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Errakhi, R.
Right arrow Articles by Bouteau, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Errakhi, R.
Right arrow Articles by Bouteau, F.
Agricola
Right arrow Articles by Errakhi, R.
Right arrow Articles by Bouteau, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Anion channel activity is necessary to induce ethylene synthesis and programmed cell death in response to oxalic acid

Rafik Errakhi1, Patrice Meimoun1, Arnaud Lehner1, Guillaume Vidal1, Joël Briand1, Françoise Corbineau2, Jean-Pierre Rona1 and François Bouteau1,*

1LEM (EA 3514), Université Paris Diderot, 2 place Jussieu, 75251 Paris cedex 05, France
2UPMC Université Paris 06, EA 2388, Physiologie des semences, Site d'Ivry, 4 place Jussieu, 75252 Paris cedex 05, France

* To whom correspondence should be addressed. E-mail: francois.bouteau{at}univ-paris-diderot.fr

Oxalic acid is thought to be a key factor of the early pathogenicity stage in a wide range of necrotrophic fungi. Studies were conducted to determine whether oxalate could induce programmed cell death (PCD) in Arabidopsis thaliana suspension cells and to detail the transduction of the signalling pathway induced by oxalate. Arabidopsis thaliana cells were treated with millimolar concentrations of oxalate. Cell death was quantified and ion flux variations were analysed from electrophysiological measurements. Involvement of the anion channel and ethylene in the signal transduction leading to PCD was determined by using specific inhibitors. Oxalic acid induced a PCD displaying cell shrinkage and fragmentation of DNA into internucleosomal fragments with a requirement for active gene expression and de novo protein synthesis, characteristic hallmarks of PCD. Other responses generally associated with plant cell death, such as anion effluxes leading to plasma membrane depolarization, mitochondrial depolarization, and ethylene synthesis, were also observed following addition of oxalate. The results show that oxalic acid activates an early anionic efflux which is a necessary prerequisite for the synthesis of ethylene and for the PCD in A. thaliana cells.

Key words: Anion channel, Arabidopsis thaliana, ethylene, oxalic acid, programmed cell death

Received 18 March 2008; Revised 13 May 2008 Accepted 14 May 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
R. Errakhi, A. Dauphin, P. Meimoun, A. Lehner, D. Reboutier, P. Vatsa, J. Briand, K. Madiona, J. P. Rona, M. Barakate, et al.
An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells
J. Exp. Bot., November 17, 2008; (2008) ern267v1.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.