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JXB Advance Access originally published online on July 24, 2008
Journal of Experimental Botany 2008 59(12):3347-3357; doi:10.1093/jxb/ern184
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Potato skin proteome is enriched with plant defence components

Gilli Barel and Idit Ginzberg*

Institute of Plant Sciences, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel

* To whom correspondence should be addressed. E-mail: iditgin{at}volcani.agri.gov.il

Periderm is a tissue of secondary origin that replaces damaged epidermis. It can be found in underground plant organs, as an above-ground tissue of woody species (cork), and as a wound-healing tissue. Its outer layers are composed of phellem cells with suberized walls that constitute a protective barrier, preventing pathogen invasion and fluid loss. In potato, a model for periderm studies, periderm tissue replaces the epidermis early in tuber development and the suberized phellems constitute the tuber's skin. To identify factors involved in phellem/skin development and that play a role in its defensive characteristics, two-dimensional gel electrophoresis was used to compare the skin and parenchymatic flesh proteomes of young developing tubers. Proteins exhibiting differentially high signal intensity in the skin were sorted by functional categories. As expected, the differential skin proteome was enriched in proteins whose activity is characteristic of actively dividing tissues such as cell proliferation, C1 metabolism, and the oxidative respiratory chain. Interestingly, the major functional category consisted of proteins (63%) involved in plant defence responses to biotic and abiotic stresses. This group included three isozymes of caffeoyl-CoA O-methyltransferase and five isozymes of peroxidase that may play a role in suberization processes. The differential expression of these proteins in the skin was further verified by RT-PCR of their corresponding transcripts in skin and tuber flesh samples. The results presented here shed light on the early events in skin development and further expand the concept of the periderm as a protective tissue containing an array of plant defence components.

Key words: Periderm, potato tuber, Solanum tuberosum, suberization

Received 24 December 2007; Revised 3 June 2008 Accepted 24 June 2008


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I. Ginzberg, G. Barel, R. Ophir, E. Tzin, Z. Tanami, T. Muddarangappa, W. de Jong, and E. Fogelman
Transcriptomic profiling of heat-stress response in potato periderm
J. Exp. Bot., November 1, 2009; 60(15): 4411 - 4421.
[Abstract] [Full Text] [PDF]


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