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JXB Advance Access originally published online on July 22, 2008
Journal of Experimental Botany 2008 59(12):3371-3381; doi:10.1093/jxb/ern187
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi

Andreia Figueiredo1,*, Ana Margarida Fortes1, Silvia Ferreira1, Mónica Sebastiana1, Young Hae Choi2, Lisete Sousa3, Bartolomeu Acioli-Santos1 {dagger}, Fernando Pessoa1, Robert Verpoorte2 and Maria Salomé Pais1

1Unit of Molecular Biology and Plant Biotechnology, ICAT, FCUL, Campo Grande, 1749-016 Lisboa, Portugal
2Division of Pharmacognosy, Section Metabolomics, Institute of Biology Leiden University, Leiden, The Netherlands
3Department of Statistics and Operational Research, FCUL, CEAUL, Lisboa, Portugal

* To whom correspondence should be addressed. E-mail: figueiredo.andreia{at}gmail.com or aafigueiredo{at}fc.ul.pt

Grapevine species (Vitis sp.) are prone to several diseases, fungi being the major pathogens compromising its cultivation and economic profit around the world. Knowledge of the complexity of mechanisms responsible for resistance to fungus infection of cultivars, such as Regent, is necessary for strategies to be defined which will improve resistance in highly susceptible crop species. Transcript and metabolic profiles of the Vitis vinifera cultivars Regent and Trincadeira (resistant and susceptible to fungi, respectively) were analysed by cDNA microarray, quantitative real-time PCR, and nuclear magnetic resonance spectroscopy. The integration of datasets obtained through transcriptome and metabolome analysis revealed differences in transcripts and metabolites between both cultivars. These differences are probably associated with the innate resistance of Regent towards the mildews. Several transcripts related to stress and defence, namely a subtilisin-like protease, phenylalanine ammonia lyase, S-adenosylmethionine synthase, WD-repeat protein like, and J2P, were up-regulated in Regent suggesting an intrinsic resistance capability of this cultivar. A metabolic profile revealed an accumulation of compounds such as inositol and caffeic acid, which are known to confer resistance to fungi. The differences in transcripts and metabolites detected are discussed in terms of the metabolic pathways and their possible role in plant defence against pathogen attack, as well as their potential interest to discriminate among resistant and susceptible grapevine cultivars.

Key words: cDNA microarrays, NMR-based metabolomics, Regent, Vitis vinifera

{dagger} Present address: Dep. Micologia, Univ. Federal de Pernambuco. Av. Prof. Nelson Chaves s/n, Cidade Universitária, 50670-420, Recife, PE, Brazil.

Received 24 March 2008; Revised 20 June 2008 Accepted 24 June 2008


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