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JXB Advance Access originally published online on August 25, 2008
Journal of Experimental Botany 2008 59(13):3587-3594; doi:10.1093/jxb/ern208
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Phospholipase A2β mediates light-induced stomatal opening in Arabidopsis

Jiyoung Seo1 *, Hyoung Yool Lee2 *, Hyunju Choi1, Yunjung Choi1, Yuree Lee1, Yong-Woo Kim3, Stephen Beungtae Ryu2,{dagger} and Youngsook Lee1,{dagger}

1Division of Molecular and Life Sciences, POSTECH, Pohang 790-784, Korea
2Bio-evaluation Center, KRIBB, Ochang, Chungbuk 363-883, Korea
3Center for Plant Intracellular Trafficking, POSTECH, Pohang, 790-784, Korea

{dagger} To whom correspondence should be addressed. E-mails: ylee{at}postech.ac.kr; sbryu{at}kribb.re.kr

Phospholipase A2 (PLA2) catalyses the hydrolysis of phospholipids into lysophospholipids and free fatty acids. Physiological studies have indicated that PLA2 is involved in stomatal movement. However, genetic evidence of a role of PLA2 in guard cell signalling has not yet been reported. To identify PLA2 gene(s) that is (are) involved in light-induced stomatal opening, stomatal movement was examined in Arabidopsis thaliana plants in which the expression of PLA2 isoforms was reduced or knocked-out. Light-induced stomatal opening in PLA2{alpha} knockout plants did not differ from wild-type plants. Plants in which PLA2β was silenced by RNA interference exhibited delayed light-induced stomatal opening, and this phenotype was reversed by exogenous lysophospholipids, which are products of PLA2. Stomatal opening in transgenic plants that over-expressed PLA2β was faster than wild-type plants. The expression of PLA2β was localized to the endoplasmic reticulum of guard cells, and increased in response to light in the mature leaf. Aristolochic acid, which inhibits light-induced stomatal opening, inhibited the activity of purified PLA2β. Collectively, these results provide evidence that PLA2β is involved in light-induced stomatal opening in Arabidopsis.

Key words: Guard cell, light signal transduction, phospholipase A2, stomata

* These authors contributed equally to the work.

Received 30 March 2008; Revised 10 July 2008 Accepted 21 July 2008


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