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JXB Advance Access originally published online on February 10, 2008
Journal of Experimental Botany 2008 59(3):545-552; doi:10.1093/jxb/erm339
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Glycosylation of S-RNases may influence pollen rejection thresholds in Solanum chacoense

Bolin Liu, David Morse and Mario Cappadocia*

IRBV, Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke est, Montréal, Québec, Canada H1X 2B2

* To whom correspondence should be addressed. E-mail: mario.cappadocia{at}umontreal.ca

A survey of Solanum chacoense plants expressing an authentic S11-RNase transgene identified a line with partial compatibility to S11 pollen. By comparing fruit set to the S-RNase levels determined immunologically in single styles, the minimum level of S11-RNase required for full rejection of S11 pollen was estimated to be 18 ng per style. The S11-RNase threshold levels are thus considerably lower than those previously reported for the S12-RNase. Interestingly, these two allelic S-RNases differ dramatically in the extent of glycosylation, with the number of glycosylation sites varying from one (S11-RNase) to four (S12-RNase). It is suggested that reduced glycosylation of the S11-RNase may be related to the lower threshold for pollen rejection.

Key words: Gametophytic self-incompatibility, glycosylation, pistil-by-pistil analysis, S-RNase, Solanum chacoense, threshold

Received 13 August 2007; Revised 27 November 2007 Accepted 30 November 2007


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