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JXB Advance Access originally published online on February 4, 2008
Journal of Experimental Botany 2008 59(3):563-573; doi:10.1093/jxb/erm331
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Jasmonate-induced transcriptional changes suggest a negative interference with the ripening syndrome in peach fruit

Vanina Ziosi1, Claudio Bonghi2, Anna Maria Bregoli1, Livio Trainotti3, Stefania Biondi4, Setha Sutthiwal5, Satoru Kondo5, Guglielmo Costa1 and Patrizia Torrigiani4,*

1Dipartimento di Colture Arboree, Università di Bologna, Via Fanin 46, 40127 Bologna, Italy
2Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Agripolis, Università di Padova, Italy
3Dipartimento di Biologia, Università di Padova, Via G. Colombo 3, 35121 Padova, Italy
4Dipartimento di Biologia e.s., Università di Bologna, Via Irnerio 42, 40126 Bologna, Italy
5Graduate School of Horticulture, Chiba University, Matsudo 648, Matsudo, Chiba, Japan

* To whom correspondence should be addressed. E-mail: patrizia.torrigiani{at}unibo.it

Peach (Prunus persica L. Batsch) was chosen as a model to shed light on the physiological role of jasmonates (JAs) during fruit ripening. To this aim, the effects of methyl jasmonate (MJ, 0.40 mM) and propyl dihydrojasmonate (PDJ, 0.22 mM), applied in planta at different fruit developmental stages, on the time-course of ethylene production and fruit quality traits were evaluated. MJ-induced changes in fruit transcriptome at harvest and the expression profiling of relevant JA-responsive genes were analysed in control and JA-treated fruit. Exogenously applied JAs affected the onset of ripening depending upon the fruit developmental stage, with PDJ being more active than MJ. Both compounds enhanced the transcription of allene oxide synthase (PpAOS1), the first specific enzyme in the biosynthesis of jasmonic acid, and altered the pattern of jasmonic acid accumulation. Microarray transcriptome profiling showed that MJ down-regulated some ripening-related genes, such as 1-aminocyclopropane-1-carboxylic acid oxidase (PpACO1) and polygalacturonase (PG), and the transcriptional modulator IAA7. MJ also altered the expression of cell wall-related genes, namely pectate lyase (PL) and expansins (EXPs), and up-regulated several stress-related genes, including some of those involved in JA biosynthesis. Time-course expression profiles of PpACO1, PL, PG, PpExp1, and the transcription factor LIM confirmed the array results. Thus, in peach fruit, exogenous JAs led to a ripening delay due to an interference with ripening- and stress/defence-related genes, as reflected in the transcriptome of treated fruit at harvest.

Key words: Allene oxide synthase, ethylene, fruit ripening, jasmonic acid, methyl jasmonate, microarray, propyl dihydrojasmonate, Prunus persica

Received 14 September 2007; Revised 9 November 2007 Accepted 27 November 2007


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