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JXB Advance Access originally published online on February 27, 2008
Journal of Experimental Botany 2008 59(4):779-791; doi:10.1093/jxb/erm363
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes

Céline Richard-Molard1,*, Anne Krapp2, François Brun1, Bertrand Ney1, Françoise Daniel-Vedele2 and Sylvain Chaillou2

1INRA, UMR1091 Environnement et Grandes Cultures, F-78850 Thiverval Grignon, France
2INRA, UR511 Nutrition Azotée des Plantes, F-7800 Versailles, France

* To whom correspondence should be addressed. E-mail: richardm{at}grignon.inra.fr

In a low-input agricultural context, plants facing temporal nutrient deficiencies need to be efficient. By comparing the effects of NOFormula-starvation in two lines of Arabidopsis thaliana (RIL282 and 432 from the Bay-0xShahdara population), this study aimed to screen the physiological mechanisms allowing one genotype to withstand NOFormula-deprivation better than another and to rate the relative importance of processes such as nitrate uptake, storage, and recycling. These two lines, chosen because of their contrasted shoot N contents for identical shoot biomass under N-replete conditions, underwent a 10 d nitrate starvation after 28 d of culture at 5 mM NOFormula. It was demonstrated that line 432 coped better with NOFormula-starvation, producing higher shoot and root biomass and sustaining maximal growth for a longer time. However, both lines exhibited similar features under NOFormula-starvation conditions. In particular, the nitrate pool underwent the same drastic and early depletion, whereas the protein pool was increased to a similar extent. Nitrate remobilization rate was identical too. It was proportional to nitrate content in both shoots and roots, but it was higher in roots. One difference emerged: line 432 had a higher nitrate content at the beginning of the starvation phase. This suggests that to overcome NOFormula-starvation, line 432 did not directly rely on the N pool composition, nor on nitrate remobilization efficiency, but on higher nitrate storage capacities prior to NOFormula-starvation. Moreover, the higher resistance of 432 corresponded to a higher nitrate uptake capacity and a 2–9-fold higher expression of AtNRT1.1, AtNRT2.1, and AtNRT2.4 genes, suggesting that the corresponding nitrate transporters may be preferentially involved under fluctuating N supply conditions.

Key words: Arabidopsis thaliana, genetic variability, N partitioning, N recycling, N use efficiency, nitrate deficiency, nitrate remobilization rate, nitrate transporter gene expression, nitrogen reserves, plant development

Received 12 July 2007; Revised 21 November 2007 Accepted 17 December 2007


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