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JXB Advance Access originally published online on February 13, 2008
Journal of Experimental Botany 2008 59(4):815-825; doi:10.1093/jxb/erm370
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba

Zhenfeng An, Wen Jing, Youliang Liu and Wenhua Zhang*

College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, PR China

* To whom correspondence should be addressed. E-mail: whzhang{at}njau.edu.cn

H2O2 is an essential signal in absicic acid (ABA)-induced stomatal closure. It can be synthesized by several enzymes in plants. In this study, the roles of copper amine oxidase (CuAO) in H2O2 production and stomatal closure were investigated. Exogenous ABA stimulated apoplast CuAO activity, increased H2O2 production and [Ca2+]cyt levels in Vicia faba guard cells, and induced stomatal closure. These processes were impaired by CuAO inhibitor(s). In the metabolized products of CuAO, only H2O2 could induce stomatal closure. By the analysis of enzyme kinetics and polyamine contents in leaves, putrescine was regarded as a substrate of CuAO. Putrescine showed similar effects with ABA on the regulation of H2O2 production, [Ca2+]cyt levels, as well as stomatal closure. The results suggest that CuAO in V. faba guard cells is an essential enzymatic source for H2O2 production in ABA-induced stomatal closure via the degradation of putrescine. Calcium messenger is an important intermediate in this process.

Key words: Abscisic acid, calcium, copper amine oxidase, hydrogen peroxide, putrescine, stomatal closure, Vicia faba

Received 13 October 2007; Revised 16 December 2007 Accepted 20 December 2007


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