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JXB Advance Access originally published online on March 28, 2008
Journal of Experimental Botany 2008 59(6):1341-1351; doi:10.1093/jxb/ern041
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Phloem transdifferentiation from immature xylem cells during bark regeneration after girdling in Eucommia ulmoides Oliv

Yu Pang, Jing Zhang, Jing Cao, Shen-Yi Yin, Xin-Qiang He* and Ke-Ming Cui*

Peking University, College of Life Sciences, Beijing 100871, PR China

* To whom correspondence should be addressed. E-mail: hexq{at}pku.edu.cn, ckm{at}pku.edu.cn

Eucommia ulmoides Oliv. (Eucommiaceae), a traditional Chinese medicinal plant, was used to study phloem cell differentiation during bark regeneration after girdling on a large scale. Here it is shown that new sieve elements (SEs) appeared in the regenerated tissues before the formation of wound cambium during bark regeneration after girdling, and they could originate from the transdifferentiation of immature/differentiating axial xylem cells left on the trunk. Assays of water-cultured twigs revealed that girdling blocked sucrose transport until the formation of new SEs, and the regeneration of the functional SEs was not dependent on the substance provided by the axis system outside the girdled areas, while exogenous indole acetic acid (IAA) applied on the wound surface accelerated SE differentiation. The experiments suggest that the immature xylem cells can transdifferentiate into phloem cells under certain conditions, which means xylem and phloem cells might share some identical features at the beginning of their differentiation pathway. This study also showed that the bark regeneration system could provide a novel method for studying xylem and phloem cell differentiation.

Key words: Bark regeneration, Eucommia ulmoides Oliv., immature xylem cells, sieve elements, transdifferentiation

Received 19 November 2007; Revised 23 January 2008 Accepted 24 January 2008


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