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Journal of Experimental Botany 2008 59(9):2379-2392; doi:10.1093/jxb/ern111
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© The Author [2008]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Differential transcript accumulation in Cicer arietinum L. in response to a chewing insect Helicoverpa armigera and defence regulators correlate with reduced insect performance

Archana Singh1 *, Indrakant Kumar Singh2 * and Praveen Kumar Verma1,{dagger}

1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, JNU Campus, New Delhi 110 067, India
2Centre of Advanced Study in Zoology, Department of Zoology, University of Delhi, Delhi 110 007, India

{dagger} To whom correspondence should be addressed. E-mail: praveenverma{at}india.com

Monitoring transcriptional reorganization triggered in response to a particular stress is an essential first step for the functional analysis of genes involved in the process. To characterize Cicer arietinum L. defence responses against Helicoverpa armigera feeding, transcript patterns elicited by both herbivore and mechanical wounding were profiled and compared, and the application of defence regulators was assessed. A combination of approaches was employed to develop transcript profiles, including suppression subtractive hybridization (SSH), macroarray, northern blot, and cluster analysis. Of the 63 unique genes isolated, 29 genes expressed differentially when Helicoverpa feeding and wounding responses were compared. Comparative macroarray analyses revealed that most of the Helicoverpa-induced transcripts were methyl jasmonate (MeJA) and ethylene (ET) regulated. The effects of mild insect infestation and the exogenous application of signalling compounds on larval feeding behaviour were also monitored. Bioassays were performed to measure dispersal percentage and growth of larvae on elicited plants. Larvae released on elicited plants had decreased larval performance, demonstrating the central role of induced plant defence against herbivory. Similarly, wounding and exogenous application of MeJA and ET also affected larval growth and feeding behaviour. Our results demonstrated that Helicoverpa attack up-regulated large transcriptional changes and induced chickpea defence responses. Therefore, the results of this study advance the understanding of non-model plant–insect interactions on a broader scale.

Key words: Chickpea, ET, Helicoverpa, induced plant defence, MeJA, SA, SSH

* These authors contributed equally to the paper.

Received 29 January 2008; Revised 12 March 2008 Accepted 17 March 2008


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