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JXB Advance Access originally published online on August 11, 2009
Journal of Experimental Botany 2009 60(14):4003-4014; doi:10.1093/jxb/erp241
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© The Author [2009]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Rubisco activase and wheat productivity under heat-stress conditions

Zoran Ristic1,*, Ivana Momcilovic2 {dagger}, Urska Bukovnik2 {ddagger}, P. V. Vara Prasad2, Jianming Fu2, Benjamin P. DeRidder3, Thomas E. Elthon4 and Novica Mladenov5

1United States Department of Agriculture–Agricultural Research Service, Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS 66506, USA
2Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA
3Department of Biology, Grinnell College, Grinnell, IA 50112, USA
4Department of Agronomy and Horticulture, Plant Science Initiative, Center for Biotechnology, and School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA
5Institute of Field and Vegetable Crops, Novi Sad, Serbia

* To whom correspondence should be addressed: E-mail: zoran.ristic{at}ars.usda.gov

Rubisco activase (RCA) constrains the photosynthetic potential of plants at high temperatures (heat stress). Endogenous levels of RCA could serve as an important determinant of plant productivity under heat-stress conditions. Thus, in this study, the possible relationship between expression levels of RCA and plant yield in 11 European cultivars of winter wheat following prolonged exposure to heat stress was investigated. In addition, the effect of a short-term heat stress on RCA expression in four genotypes of wheat, five genotypes of maize, and one genotype of Arabidopsis thaliana was examined. Immunoblots prepared from leaf protein extracts from control plants showed three RCA cross-reacting bands in wheat and two RCA cross-reacting bands in maize and Arabidopsis. The molecular mass of the observed bands was in the range between 40 kDa and 46 kDa. Heat stress affected RCA expression in a few genotypes of wheat and maize but not in Arabidopsis. In wheat, heat stress slightly modulated the relative amounts of RCA in some cultivars. In maize, heat stress did not seem to affect the existing RCA isoforms (40 kDa and 43 kDa) but induced the accumulation of a new putative RCA of 45–46 kDa. The new putative 45–46 kDa RCA was not seen in a genotype of maize (ZPL 389) that has been shown to display an exceptional sensitivity to heat stress. A significant, positive, linear correlation was found between the expression of wheat 45–46 kDa RCA and plant productivity under heat-stress conditions. Results support the hypothesis that endogenous levels of RCA could play an important role in plant productivity under supraoptimal temperature conditions.

Key words: Heat stress, heat tolerance, Rubisco activase, wheat productivity

{dagger} Present address: Department of Plant Physiology, Institute for Biological Research, 11060 Belgrade, Serbia.

{ddagger} Present address: Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.

Received 13 February 2009; Revised 30 June 2009 Accepted 3 July 2009


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