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JXB Advance Access originally published online on August 25, 2009
Journal of Experimental Botany 2009 60(14):4201-4213; doi:10.1093/jxb/erp254
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Molecular dissection of the pea shoot apical meristem*

Dacheng Liang1 {dagger}, Chui E. Wong1 {dagger}, Mohan B. Singh1, Christine A. Beveridge2, Belinda Phipson3, Gordon K. Smyth3 and Prem L. Bhalla1,{ddagger}

1Plant Molecular Biology and Biotechnology Laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, University of Melbourne, Parkville, Victoria 3010, Australia
2Australian Research Council Centre of Excellence for Integrative Legume Research, University of Queensland, Brisbane St Lucia, QLD 4072, Australia
3Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia

{ddagger} To whom correspondence should be addressed: E-mail: premlb{at}unimelb.edu.au

The shoot apical meristem (SAM) is responsible for the development of all the above-ground parts of a plant. Our understanding of the SAM at the molecular level is incomplete. This study investigates the gene expression repertoire of SAMs in the garden pea (Pisum sativum). To this end, 10 346 EST sequences representing 7610 unique genes were generated from SAM cDNA libraries. These sequences, together with previously reported pea ESTs, were used to construct a 12K oligonucleotide array to identify genes with differential SAM expression, as compared to axillary meristems, root apical meristems, or non-meristematic tissues. A number of genes were identified, predominantly expressed in specific cell layers or domains of the SAM and thus are likely components of the gene networks involved in stem cell maintenance or the initiation of lateral organs. Further in situ hybridization analysis confirmed the spatial localization of some of these genes within the SAM. Our data also indicate the diversification of some gene expression patterns and hence functions in legume crop plants. A number of transcripts highly expressed in all three meristems have also been uncovered and these candidates may provide valuable insight into molecular networks that underpin the maintenance of meristematic functionality.

Key words: Garden pea, meristem, Pisum sativum, transcript profiling

* The EST sequences in this paper have been submitted to GenBank under accession numbers FG528667 to FG539012. The microarray data have been submitted to GEO (http://www.ncbi.nlm.gov/geo/) under the accession GSE13451.

{dagger} These authors contributed equally to this work.

Received 26 May 2009; Revised 31 July 2009 Accepted 31 July 2009


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