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JXB Advance Access originally published online on August 28, 2009
Journal of Experimental Botany 2009 60(15):4249-4262; doi:10.1093/jxb/erp256
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© The Author [2009]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress

Andrés Alberto Rodríguez1 *,{dagger}, Santiago Javier Maiale1 *, Ana Bernardina Menéndez1,2 and Oscar Adolfo Ruiz1

1Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas–Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas–Universidad Nacional de General San Martín (IIB-INTECH/CONICET-UNSAM), Camino de Circunvalación Laguna, Km 6 CC 164 (B7130IWA) Chascomús, Argentina
2Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, DBBE, Piso 4, Pab II, Ciudad Universitaria (1428), Ciudad Autónoma de Buenos Aires

{dagger} To whom correspondence should be addressed: E-mail: andresrodriguez{at}intech.gov.ar

The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxidase was up to 20-fold higher than that of the copper-containing amine oxidase. Measurements of H2O2, ·OFormula, and HO· production in the presence or absence of the polyamine oxidase inhibitors 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane suggest that, in salinized plants, the oxidation of free apoplastic polyamines by polyamine oxidase by would be the main source of reactive oxygen species in the elongation zone of maize leaf blades. This effect is probably due to increased substrate availability. Incubation with 200 µM spermine doubled segment elongation, whereas the addition of 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane to 200 µM spermine attenuated and reversed the last effect, respectively. Similarly, the addition of MnCl2 (an ·OFormula dismutating agent) or the HO· scavenger sodium benzoate along with spermine, annulled the elongating effect of the polyamine on the salinized segments. As a whole, the results obtained here demonstrated that, under salinity, polyamine oxidase activity provides a significant production of reactive oxygen species in the apoplast which contributes to 25–30% of the maize leaf blade elongation.

Key words: Growth, maize, polyamine oxidase, polyamines, reactive oxygen species, salinity

* Both authors contributed equally to this work.

Received 3 June 2009; Revised 28 July 2009 Accepted 6 August 2009


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