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JXB Advance Access originally published online on March 30, 2009
Journal of Experimental Botany 2009 60(6):1849-1857; doi:10.1093/jxb/erp065
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© The Author [2009]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Proteome changes induced by aluminium stress in tomato roots

Suping Zhou1,*, Roger Sauvé1 and Theodore W. Thannhauser2

1Department of Agricultural Sciences, School of Agriculture and Consumer Sciences, Tennessee State University, 3500 John A Merritt Blvd, Nashville, TN 37209, USA
2USDA-ARS, 102A R.W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, USA

* To whom correspondence should be addressed: E-mail: zsuping{at}tnstate.edu

Growth inhibition in acid soils due to Al stress affects crop production worldwide. To understand mechanisms in sensitive crops that are affected by Al stress, a proteomic analysis of primary tomato root tissue, grown in Al-amended and non-amended liquid cultures, was performed. DIGE-SDS-MALDI-TOF-TOF analysis of these tissues resulted in the identification of 49 proteins that were differentially accumulated. Dehydroascorbate reductase, glutathione reductase, and catalase enzymes associated with antioxidant activities were induced in Al-treated roots. Induced enzyme proteins associated with detoxification were mitochondrial aldehyde dehydrogenase, catechol oxidase, quinone reductase, and lactoylglutathione lyase. The germin-like (oxalate oxidase) proteins, the malate dehydrogenase, wali7 and heavy-metal associated domain-containing proteins were suppressed. VHA-ATP that encodes for the catalytic subunit A of the vacuolar ATP synthase was induced and two ATPase subunit 1 isoforms were suppressed. Several proteins in the active methyl cycle, including SAMS, quercetin 3-O-methyltransferase and AdoHcyase, were induced by Al stress. Other induced proteins were isovaleryl-CoA dehydrogenase and the GDSL-motif lipase hydrolase family protein. NADPH-dependent flavin reductase and β-hydroxyacyl-ACP dehydratase were suppressed.

Key words: Acidic pH, aluminium toxicity, DIGE-MALDI-TOF-TOF, proteomics, tomato root

Received 4 December 2008; Revised 23 January 2009 Accepted 16 February 2009


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