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JXB Advance Access originally published online on April 8, 2009
Journal of Experimental Botany 2009 60(7):2021-2034; doi:10.1093/jxb/erp079
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Expression patterns of cell wall-modifying genes from banana during fruit ripening and in relationship with finger drop

D. Mbéguié-A-Mbéguié1,*, O. Hubert2,6, F. C. Baurens3, T. Matsumoto4, M. Chillet5,6,7, B. Fils-Lycaon8 and S. Sidibé-Bocs3

1CIRAD, UMR QUALITROP, F-97130 Capesterre-Belle-Eau, Guadeloupe France
2CIRAD, UMR QUALISUD, F-97130 Capesterre-Belle-Eau, Guadeloupe, France
3CIRAD, UMR DAP, F-34398 Montpellier, France
4Rice Genome Research Program (RGP), National Institute of Agrobiological Sciences (NIAS)/Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-8602, Japan
5CIRAD, UMR QUALISUD, Sao Paulo, SP, Brazil
6CIRAD, UMR QUALISUD, F-34398 Montpellier, France
7Universidade de Sao Paulo, Dpto dos Alimentos e Nutriçao Experimental USP/FCF, São Paulo, SP, Brazil
8INRA, UMR QUALITROP, F-97170 Petit-Bourg, Guadeloupe, France

* To whom correspondence should be addressed. didier.mbeguie-a-mbeguie{at}cirad.fr

Few molecular studies have been devoted to the finger drop process that occurs during banana fruit ripening. Recent studies revealed the involvement of changes in the properties of cell wall polysaccharides in the pedicel rupture area. In this study, the expression of cell-wall modifying genes was monitored in peel tissue during post-harvest ripening of Cavendish banana fruit, at median area (control zone) and compared with that in the pedicel rupture area (drop zone). To this end, three pectin methylesterase (PME) and seven xyloglucan endotransglycosylase/hydrolase (XTH) genes were isolated. The accumulation of their mRNAs and those of polygalaturonase, expansin, and pectate lyase genes already isolated from banana were examined. During post-harvest ripening, transcripts of all genes were detected in both zones, but accumulated differentially. MaPME1, MaPG1, and MaXTH4 mRNA levels did not change in either zone. Levels of MaPME3 and MaPG3 mRNAs increased greatly only in the control zone and at the late ripening stages. For other genes, the main molecular changes occurred 1–4 d after ripening induction. MaPME2, MaPEL1, MaPEL2, MaPG4, MaXTH6, MaXTH8, MaXTH9, MaEXP1, MaEXP4, and MaEXP5 accumulated highly in the drop zone, contrary to MaXTH3 and MaXTH5, and MaEXP2 throughout ripening. For MaPG2, MaXET1, and MaXET2 genes, high accumulation in the drop zone was transient. The transcriptional data obtained from all genes examined suggested that finger drop and peel softening involved similar mechanisms. These findings also led to the proposal of a sequence of molecular events leading to finger drop and to suggest some candidates.

Key words: Banana, cell-wall, expansin, finger drop, Musa, pectolytic genes, quality, ripening, xyloglucan endotranglucosylase/hydrolase

Received 30 October 2008; Revised 15 February 2009 Accepted 23 February 2009


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