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JXB Advance Access published online on January 17, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erl251
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Integrated Approaches to Sustain and Improve Plant Production under Drought Stress Special Issue

Overexpression of wheat Na+/H+ antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt- and drought-stress tolerance in Arabidopsis thaliana plants

Faïçal Brini1, Moez Hanin1, Imed Mezghani1, Gerald A. Berkowitz2 and Khaled Masmoudi1,*

1Plant Molecular Genetics Unit, Centre of Biotechnology of Sfax (CBS), B.P'K', 3038 Sfax, Tunisia
2Department of Plant Science, Agricultural Biotechnology Laboratory, University of Connecticut, 1390 Storrs Road, Storrs, CT 06269-4163, USA

* To whom correspondence should be addressed. E-mail: khaled.masmoudi{at}cbs.rnrt.tn

Transgenic Arabidopsis plants overexpressing the wheat vacuolar Na+/H+ antiporter TNHX1 and H+-PPase TVP1 are much more resistant to high concentrations of NaCl and to water deprivation than the wild-type strains. These transgenic plants grow well in the presence of 200 mM NaCl and also under a water-deprivation regime, while wild-type plants exhibit chlorosis and growth inhibition. Leaf area decreased much more in wild-type than in transgenic plants subjected to salt or drought stress. The leaf water potential was less negative for wild-type than for transgenic plants. This could be due to an enhanced osmotic adjustment in the transgenic plants. Moreover, these transgenic plants accumulate more Na+ and K+ in their leaf tissue than the wild-type plants. The toxic effect of Na+ accumulation in the cytosol is reduced by its sequestration into the vacuole. The rate of water loss under drought or salt stress was higher in wild-type than transgenic plants. Increased vacuolar solute accumulation and water retention could confer the phenotype of salt and drought tolerance of the transgenic plants. Overexpression of the isolated genes from wheat in Arabidopsis thaliana plants is worthwhile to elucidate the contribution of these proteins to the tolerance mechanism to salt and drought. Adopting a similar strategy could be one way of developing transgenic staple crops with improved tolerance to these important abiotic stresses.

Key words: H+-pyrophosphatase, Na+/H+ antiporter, salt and drought tolerance, sodium sequestration, transgenic Arabidopsis plants


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