JXB Advance Access published online on October 4, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm207
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
RESEARCH PAPER |
Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots
1Department of Biological Sciences, University of Alberta, Edmonton, Canada T6G 2E9
2Department of Agricultural, Forestry and Nutritional Science, University of Alberta, Edmonton, Canada T6G 2E9
* To whom correspondence should be addressed. E-mail: deyholos{at}ualberta.ca
NaCl stress is a major abiotic stress limiting the productivity and the geographical distribution of many plant species. Roots are the primary site of salinity perception. To understand better NaCl stress responses in Arabidopsis roots, a comparative proteomic analysis of roots that had been exposed to 150 mM NaCl for either 6 h or 48 h was conducted. Changes in the abundance of protein species within roots were examined using two-dimensional electrophoresis. Among the >1000 protein spots reproducibly detected on each gel, the abundance of 112 protein spots decreased and 103 increased, at one or both time points, in response to NaCl treatment. Through liquid-chromatography–tandem mass spectrometry, identity was assigned to 86 of the differentially abundant spots. The proteins identified included many previously characterized stress-responsive proteins and others related to processes including scavenging for reactive oxygen species; signal transduction; translation, cell wall biosynthesis, protein translation, processing and degradation; and metabolism of energy, amino acids, and hormones. At the resolution of individual genes and proteins, poor statistical correlation (6 h, r= –0.13; 48 h, r=0.11) of these protein expression data with previous microarray results was detected, supporting the concept that post-transcriptional regulation plays an important role in stress-responsive gene expression, and highlighting the need for combined transcriptomic and proteomic analyses.
Key words: Arabidopsis, 2-DGE, LC-MS/MS, NaCl stress, proteome, root
Received 10 May 2007; Revised 25 July 2007 Accepted 6 August 2007
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