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JXB Advance Access published online on December 7, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm271
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Tissue specialization at the metabolite level is perceived during the development of tomato fruit

Sofia Moco1,2,3 *,{dagger}, Esra Capanoglu2,4 *, Yury Tikunov2,3, Raoul J. Bino2,3,5, Dilek Boyacioglu4, Robert D. Hall2,3, Jacques Vervoort1,3 and Ric C. H. De Vos2,3

1Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
2Plant Research International, PO Box 16, 6700 AA, Wageningen, The Netherlands
3Centre for BioSystems Genomics, PO Box 98, 6700 AB Wageningen, The Netherlands
4Food Engineering Department, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, TR-34469 Istanbul, Turkey
5Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands

{dagger} To whom correspondence should be adrressed. E-mail: sofia.moco{at}wur.nl

Fruit maturation and tissue differentiation are important topics in plant physiology. These biological phenomena are accompanied by specific alterations in the biological system, such as differences in the type and concentration of metabolites. The secondary metabolism of tomato (Solanum lycopersicum) fruit was monitored by using liquid chromatography (LC) coupled to photo-diode array (PDA) detection, fluorescence detection (FD), and mass spectrometry (MS). Through this integrated approach different classes of compounds were analysed: carotenoids, xanthophylls, chlorophylls, tocopherols, ascorbic acid, flavonoids, phenolic acids, glycoalkaloids, saponins, and other glycosylated derivatives. Related metabolite profiles of peel and flesh were found between several commercial tomato cultivars indicating similar metabolite trends despite the genetic background. For a single tomato cultivar, metabolite profiles of different fruit tissues (vascular attachment region, columella and placenta, epidermis, pericarp, and jelly parenchyma) were examined at the green, breaker, turning, pink, and red stages of fruit development. Unrelated to the chemical nature of the metabolites, behavioural patterns could be assigned to specific ripening stages or tissues. These findings suggest spatio-temporal specificity in the accumulation of endogenous metabolites from tomato fruit.

Key words: Fluorescence detection, fruit tissues, liquid chromatography, mass spectrometry, metabolomics, photo-diode array, ripening, tomato fruit

* These authors contributed equally to this work.

Received 30 August 2007; Revised 4 October 2007 Accepted 8 October 2007


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