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JXB Advance Access published online on February 7, 2008
Journal of Experimental Botany, doi:10.1093/jxb/erm301
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© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Proximal–distal patterns of transcription factor gene expression during Arabidopsis root development

Paul Derbyshire, Sinéad Drea *, Peter J. Shaw, John H. Doonan and Liam Dolan{dagger}

Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK

{dagger} To whom correspondence should be addressed. E-mail: liam.dolan{at}bbsrc.ac.uk

The expression pattern of genes can identify the cells in which the respective proteins are active during development. As a step towards defining the genetic network that controls the development of roots, a high-throughput method of whole-mount in situ hybridization has been developed that does not require expensive equipment and allows the definition of the expression patterns of 137 transcription factor genes in young developing roots. Of the 137 transcription factors, 81.8% were expressed in the root while 18.2% showed no detectable expression. In all three proximal distal zones (meristem, elongation, and differentiation) of the root, 52.6% were expressed whereas 21.2% were expressed in only two zones. Eight percent of the genes were expressed in a single proximal distal zone. Cell-specific gene expression patterns were also detected. This rapid approach identified potential key regulators of cell differentiation and provides important spatial information for the expression patterns of a large number of transcriptional regulators that function during root development.

Key words: Arabidopsis thaliana, expression profiles, root development, transcription factors

* Present address Department of Molecular, Cellular and Developmental Biology, PO Box 208104, Yale University, 266 Whitney Ave, New Haven, CT 06520-8104, USA.

Received 4 September 2007; Revised 6 November 2007 Accepted 7 November 2007


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